Detailed information of TA system TA020014

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/-
• Location: 263888..264567
• Replicon: chromosome
• Accession: CP009789
• Organism: Escherichia coli K-12 strain ER3413

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: ER3413_249
• Protein ID: AIZ52221.1
• Coordinates: 263888..264229 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: -
• Locus tag: ER3413_250
• Protein ID: AIZ52233.1
• Coordinates: 264250..264567 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ER3413_244	259165..259566	+	402	AIZ52176.1	sigma factor-binding protein, stimulates RNA polymerase holoenzyme formation	-
ER3413_245	259605..260660	-	1056	AIZ52187.1	outer membrane phosphoporin protein E	-
ER3413_246	260948..262051	+	1104	AIZ52197.1	gamma-glutamate kinase	-
ER3413_247	262063..263316	+	1254	AIZ52208.1	gamma-glutamylphosphate reductase	-
ER3413_249	263888..264229	-	342	AIZ52221.1	CP4-6 prophage; toxin of the YkfI-YafW toxin-antitoxin system	Toxin
ER3413_250	264250..264567	-	318	AIZ52233.1	CP4-6 prophage; antitoxin of the YkfI-YafW toxin-antitoxin system	Antitoxin
ER3413_251	264586..264807	-	222	AIZ52244.1	hypothetical protein	-
ER3413_252	264816..265292	-	477	AIZ52255.1	CP4-6 prophage; predicted DNA repair protein	-
ER3413_253	265308..265766	-	459	AIZ52265.1	CP4-6 prophage; putative protein	-
ER3413_254	265864..266103	-	240	AIZ52276.1	CP4-6 prophage; putative protein	-
ER3413_255	266180..266647	-	468	AIZ52287.1	CP4-6 prophage; putative protein	-
ER3413_256	266670..267113	-	444	AIZ52298.1	lipoprotein, inner membrane; overproduction stimulates degP expression; CP4-6 prophage	-
ER3413_258	267744..268565	-	822	AIZ52318.1	CP4-6 prophage; conserved protein	-
ER3413_259	268657..269520	-	864	AIZ52327.1	CP4-6 prophage; predicted GTP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T20014 AIZ52221.1 CP009789:c264229-263888 [Escherichia coli K-12]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T20014 CP009789:c264229-263888 [Escherichia coli K-12]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT20014 AIZ52233.1 CP009789:c264567-264250 [Escherichia coli K-12]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT20014 CP009789:c264567-264250 [Escherichia coli K-12]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA