Detailed information of TA system TA200138

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 2454834..2456590
• Replicon: chromosome
• Accession: NZ_CP073729
• Organism: Burkholderia pseudomallei strain BD5

Toxin (Protein)

• Gene name: doc
• Uniprot ID: B1H5R2
• Locus tag: KEX46_RS28075
• Protein ID: WP_004524002.1
• Coordinates: 2454834..2455739 (+)
• Length: 302 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: B1H5R3
• Locus tag: KEX46_RS28080
• Protein ID: WP_004532863.1
• Coordinates: 2455736..2456590 (+)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEX46_RS28045 (KEX46_28045)	2450229..2451317	-	1089	WP_004184835.1	porin	-
KEX46_RS28050 (KEX46_28050)	2451752..2452069	+	318	WP_004184916.1	non-heme iron oxygenase ferredoxin subunit	-
KEX46_RS28055 (KEX46_28055)	2452071..2452382	+	312	WP_004532856.1	hypothetical protein	-
KEX46_RS28060 (KEX46_28060)	2452417..2453484	+	1068	WP_004195457.1	aromatic ring-hydroxylating dioxygenase subunit alpha	-
KEX46_RS28065 (KEX46_28065)	2453481..2454251	+	771	WP_004184755.1	SDR family oxidoreductase	-
KEX46_RS28070 (KEX46_28070)	2454290..2454817	+	528	WP_004524001.1	cupin domain-containing protein	-
KEX46_RS28075 (KEX46_28075)	2454834..2455739	+	906	WP_004524002.1	alpha/beta fold hydrolase	Toxin
KEX46_RS28080 (KEX46_28080)	2455736..2456590	+	855	WP_004532863.1	IclR family transcriptional regulator	Antitoxin
KEX46_RS28085 (KEX46_28085)	2456587..2457384	+	798	WP_004195447.1	SDR family oxidoreductase	-
KEX46_RS28090 (KEX46_28090)	2457377..2459050	+	1674	WP_004532795.1	thiamine pyrophosphate-binding protein	-
KEX46_RS28095 (KEX46_28095)	2459043..2459858	+	816	WP_004195445.1	aspartate dehydrogenase	-
KEX46_RS28100 (KEX46_28100)	2459884..2461374	+	1491	WP_004524006.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (54-284)

Antitoxin

• Transcription regulator IclR, C-terminal (143-265)

Sequences

Toxin

Protein

• Length: 302 a.a.
• Molecular weight: 30556.69 Da
• Isoelectric Point: 8.4944

>T200138 WP_004524002.1 NZ_CP073729:2454834-2455739 [Burkholderia pseudomallei]
MSTISTTTTEAGAAPFAALLARFPERRAAVGGAGVIGYREAGEAHARRAGCEPVVLLHGIGSGAASWVRQLDALGATRRA
LAWDAPGYGASSRVAADSPVAADYAASLAAWLDALRIERCVLVGHSLGAIMAGAFARIAGERLAGLLLISPAGGYGSAPA
DVRAGRRDSRLALLASLGPQGLAAERSANMLSAQASGAAREWVRWNMARIAPAGYAQATHLLANADLASDLAHCRGRIAV
AVGGDDAITPPAACERIARAAGVGLQVIARAGHAGYVEAPDAYSALIDAFCRQCDGSRGTP

Nucleotide

• Length: 906 bp

>T200138 NZ_CP097716:2206038-2206141 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31151.58 Da
• Isoelectric Point: 7.4022

>AT200138 WP_004532863.1 NZ_CP073729:2455736-2456590 [Burkholderia pseudomallei]
MSEPLLKDADDARNDSNYVVPGLERGLRILAEFSPREPVLGAPELSKRLGIPRTTVFRLLQTLESLGFLERADKDRNYRL
GVAVLRLGFEYLSSLELTDLGLPVIESLRDATGFTAHIVIRDGRDVVFVAKAQNAGNAFGSVRVNVGTRLPAHATTHGHV
LMGDLSLAQLRALYPERALERVTKATPETVDALFNVIRDDARRGYGIGNSAFERDISVVTAPVRNEASRIVAVVTVTVPR
PEIDAALVAEGLVEKVVRAAAELSRRLNYRSDDENTYLKALGLR

Nucleotide

• Length: 855 bp

>AT200138 NZ_CP097716:c2206179-2205908 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A095K1W1