Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 1632894..1634653 Replicon chromosome
Accession NZ_CP073723
Organism Burkholderia pseudomallei strain WC19

Toxin (Protein)


Gene name hipA Uniprot ID Q63JY0
Locus tag KEX45_RS24090 Protein ID WP_004188815.1
Coordinates 1633301..1634653 (+) Length 451 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID C4I768
Locus tag KEX45_RS24085 Protein ID WP_004536731.1
Coordinates 1632894..1633304 (+) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KEX45_RS24075 (KEX45_24075) 1629431..1631827 - 2397 WP_045588572.1 cellulose biosynthesis cyclic di-GMP-binding regulatory protein BcsB -
KEX45_RS24080 (KEX45_24080) 1632293..1632556 + 264 WP_004199789.1 hypothetical protein -
KEX45_RS24085 (KEX45_24085) 1632894..1633304 + 411 WP_004536731.1 helix-turn-helix domain-containing protein Antitoxin
KEX45_RS24090 (KEX45_24090) 1633301..1634653 + 1353 WP_004188815.1 type II toxin-antitoxin system HipA family toxin Toxin
KEX45_RS24095 (KEX45_24095) 1634810..1635262 - 453 WP_004198842.1 hypothetical protein -
KEX45_RS24100 (KEX45_24100) 1635363..1635509 - 147 WP_004199790.1 hypothetical protein -
KEX45_RS24105 (KEX45_24105) 1635679..1636593 - 915 WP_004198844.1 LysR family transcriptional regulator -
KEX45_RS24110 (KEX45_24110) 1636760..1637713 + 954 WP_004187785.1 agmatinase -
KEX45_RS24115 (KEX45_24115) 1637984..1638325 + 342 WP_004539420.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(158-408)

Antitoxin

Cro/C1-type helix-turn-helix domain

(22-71)


Sequences

Toxin        


Download         Length: 451 a.a.        Molecular weight: 49848.83 Da        Isoelectric Point: 9.6807

>T200134 WP_004188815.1 NZ_CP073723:1633301-1634653 [Burkholderia pseudomallei]
VSARRARATRLHLWMNGLPVGYWEHARDGERLVYFDEWIGDPQGRPLSLSLPFTPGNQPYRGRLVSDYFDNLLPDSEPIR
RRIAMRYRTGGTSAFALLATLGRDCVGALQMLPPDEAPDDIERIRGHALADADIARLLREVTSAPQAGRHAPLDDLRLSI
AGAQEKTALLRHRGRWLLPEGSTPTTHILKLPLGLVGNRRADMRTSVENEWLCARIVAAYGLPVARCDIAQFDDQKALVV
ERFDRRPSRDARWLLRLPQEDMCQATGTSALDKYQADGGPGIETIMEVLAGSEHARDDRRAFFAAQIVFWLLAATDGHAK
NFSIAHLPGNRYRSTPLYDVLSAHPVIGRGANQLPAQRARLAMGVRGKHIHYPLHQIRRRHWIAQGQRVGFAPADVDALI
DTLTARTADVVDAVSARLPRDFPRDVADAIFSGMLGLSARLAGDAAARAP

Download         Length: 1353 bp

>T200134 NZ_CP097716:1005560-1005967 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGAGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 14508.87 Da        Isoelectric Point: 11.8540

>AT200134 WP_004536731.1 NZ_CP073723:1632894-1633304 [Burkholderia pseudomallei]
MAILIEHEIKTLGQLRPILRGFRKSAGLTQAMLASRLGVTQQTYAQFEANPASASVERLFKVLRALDIELTLTLTQVYAA
PAGKDKGEVAKTAAGARARAGARRAVPPASAPAPSAAGRAPRPARKRAAPKKREDW

Download         Length: 411 bp

>AT200134 NZ_CP097716:1005313-1005579 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3N4FSX2

Antitoxin

Source ID Structure
AlphaFold DB A0A7V8KZI9

References