Detailed information of TA system TA200124

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3802710..3803389
• Replicon: chromosome
• Accession: NZ_CP073719
• Organism: Escherichia coli strain CX12

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: J6O05_RS18945
• Protein ID: WP_000854680.1
• Coordinates: 3803048..3803389 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: J6O05_RS18940
• Protein ID: WP_000070396.1
• Coordinates: 3802710..3803027 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O05_RS18895 (3798148)	3798148..3798969	+	822	WP_000197387.1	DUF932 domain-containing protein	-
J6O05_RS18900 (3799186)	3799186..3799887	+	702	Protein_3702	WYL domain-containing protein	-
J6O05_RS18905 (3799928)	3799928..3800164	+	237	WP_223566506.1	protein YpjK	-
J6O05_RS18910 (3800164)	3800164..3800607	+	444	WP_000649865.1	hypothetical protein	-
J6O05_RS18915 (3800630)	3800630..3801097	+	468	WP_001385283.1	protein YkfB	-
J6O05_RS18920 (3801174)	3801174..3801413	+	240	WP_000194654.1	DUF905 family protein	-
J6O05_RS18925 (3801511)	3801511..3801969	+	459	Protein_3707	antirestriction protein	-
J6O05_RS18930 (3801985)	3801985..3802461	+	477	WP_000811693.1	RadC family protein	-
J6O05_RS18935 (3802470)	3802470..3802691	+	222	WP_000691994.1	DUF987 domain-containing protein	-
J6O05_RS18940 (3802710)	3802710..3803027	+	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
J6O05_RS18945 (3803048)	3803048..3803389	+	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
J6O05_RS18950 (3803632)	3803632..3804329	+	698	WP_094096600.1	IS1-like element IS1A family transposase	-
J6O05_RS18960 (3804738)	3804738..3805991	-	1254	WP_057108326.1	glutamate-5-semialdehyde dehydrogenase	-
J6O05_RS18965 (3806003)	3806003..3807106	-	1104	WP_001285288.1	glutamate 5-kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA / rhs/PAAR / vgrG/tssI / hcp2/tssD2 / tssA / hcp1/tssD1	3780298..3845660	65362

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T200124 WP_000854680.1 NZ_CP073719:3803048-3803389 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T200124 NZ_CP097712:118924-119340 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT200124 WP_000070396.1 NZ_CP073719:3802710-3803027 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT200124 NZ_CP097712:118697-118927 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7