Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2635076..2635714 Replicon chromosome
Accession NZ_CP073719
Organism Escherichia coli strain CX12

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag J6O05_RS13145 Protein ID WP_000813794.1
Coordinates 2635538..2635714 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag J6O05_RS13140 Protein ID WP_001270286.1
Coordinates 2635076..2635492 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O05_RS13120 (2630228) 2630228..2631169 - 942 WP_001251313.1 ABC transporter permease -
J6O05_RS13125 (2631170) 2631170..2632183 - 1014 WP_057108537.1 ABC transporter ATP-binding protein -
J6O05_RS13130 (2632201) 2632201..2633346 - 1146 Protein_2573 ABC transporter substrate-binding protein -
J6O05_RS13135 (2633591) 2633591..2634997 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
J6O05_RS13140 (2635076) 2635076..2635492 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
J6O05_RS13145 (2635538) 2635538..2635714 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
J6O05_RS13150 (2635936) 2635936..2636166 + 231 WP_000494244.1 YncJ family protein -
J6O05_RS13155 (2636258) 2636258..2638219 - 1962 WP_001488038.1 23S rRNA 5-hydroxycytidine C2501 synthase -
J6O05_RS13160 (2638292) 2638292..2638828 - 537 WP_000429169.1 DNA-binding transcriptional regulator SutR -
J6O05_RS13165 (2638920) 2638920..2640095 + 1176 WP_263294461.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - sodB / mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA / espR1 / rhs/PAAR / vgrG/tssI 2243379..2681059 437680


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T200122 WP_000813794.1 NZ_CP073719:c2635714-2635538 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T200122 NZ_CP097712:c113028-112723 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT200122 WP_001270286.1 NZ_CP073719:c2635492-2635076 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT200122 NZ_CP097712:c113248-113030 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References