Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 2488307..2489043 Replicon chromosome
Accession NZ_CP073719
Organism Escherichia coli strain CX12

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag J6O05_RS12420 Protein ID WP_003026803.1
Coordinates 2488307..2488789 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag J6O05_RS12425 Protein ID WP_003026799.1
Coordinates 2488777..2489043 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O05_RS12410 (2483932) 2483932..2484393 - 462 WP_000593665.1 response regulator -
J6O05_RS12415 (2484386) 2484386..2487748 - 3363 WP_029404079.1 ATP-binding protein -
J6O05_RS12420 (2488307) 2488307..2488789 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
J6O05_RS12425 (2488777) 2488777..2489043 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
J6O05_RS12430 (2489488) 2489488..2489718 - 231 WP_004206660.1 hypothetical protein -
J6O05_RS12435 (2489732) 2489732..2489935 - 204 WP_004213596.1 HHA domain-containing protein -
J6O05_RS12440 (2489996) 2489996..2490487 - 492 WP_015065591.1 hypothetical protein -
J6O05_RS12445 (2490621) 2490621..2491655 - 1035 WP_015065590.1 hypothetical protein -
J6O05_RS12450 (2491957) 2491957..2492961 - 1005 WP_000427614.1 IS110-like element IS5075 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - sodB / mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA / espR1 / rhs/PAAR / vgrG/tssI 2243379..2681059 437680


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T200121 WP_003026803.1 NZ_CP073719:c2488789-2488307 [Escherichia coli]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T200121 NZ_CP097712:68614-69012 [Escherichia coli]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACGATGAAGAACAAACCCGCCAGCGTCAGGGAGCGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGTTCGGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGTAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCCGCAGCCACACACAGC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGGCCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGAGAATTGAAGACTGGAGCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT200121 WP_003026799.1 NZ_CP073719:c2489043-2488777 [Escherichia coli]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT200121 NZ_CP097712:68387-68614 [Escherichia coli]
ATGGAAACCACCGTATTTTTCAGCAACCGCAGCCAGGCGGTCAGACTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCAGAATCATTACGCCAGCCGGAGAGACATGGGACGAATGGTTCGACGGAC
ACAACGTCAGCGCCGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References