Detailed information of TA system TA200120

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2429869..2431457
• Replicon: chromosome
• Accession: NZ_CP073719
• Organism: Escherichia coli strain CX12

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A080IXU4
• Locus tag: J6O05_RS12075
• Protein ID: WP_021574605.1
• Coordinates: 2430135..2431457 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: J6O05_RS12070
• Protein ID: WP_001613070.1
• Coordinates: 2429869..2430135 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O05_RS12065 (2426184)	2426184..2429711	+	3528	Protein_2358	ECSE_1600 family autotransporter	-
J6O05_RS12070 (2429869)	2429869..2430135	+	267	WP_001613070.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
J6O05_RS12075 (2430135)	2430135..2431457	+	1323	WP_021574605.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
J6O05_RS12080 (2431754)	2431754..2432560	-	807	WP_016239971.1	hypothetical protein	-
J6O05_RS12085 (2432578)	2432578..2433018	-	441	WP_016239970.1	hypothetical protein	-
J6O05_RS12090 (2433171)	2433171..2434532	-	1362	WP_016239969.1	FRG domain-containing protein	-
J6O05_RS12095 (2434546)	2434546..2435349	-	804	WP_000589340.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	sodB / mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA / espR1 / rhs/PAAR / vgrG/tssI	2243379..2681059	437680
	-	flank	IS/Tn	-	-	2435408..2436766	1358

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49304.94 Da
• Isoelectric Point: 8.5823

>T200120 WP_021574605.1 NZ_CP073719:2430135-2431457 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T200120 NZ_CP097712:c59658-59509 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGAGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10033.36 Da
• Isoelectric Point: 4.9632

>AT200120 WP_001613070.1 NZ_CP073719:2429869-2430135 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT200120 NZ_CP097712:59702-59763 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A080IXU4