Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1082809..1083392 Replicon chromosome
Accession NZ_CP073719
Organism Escherichia coli strain CX12

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag J6O05_RS05280 Protein ID WP_000254739.1
Coordinates 1083057..1083392 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag J6O05_RS05275 Protein ID WP_000581937.1
Coordinates 1082809..1083057 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O05_RS05265 (1079148) 1079148..1080449 + 1302 WP_000046826.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
J6O05_RS05270 (1080497) 1080497..1082731 + 2235 WP_000226803.1 GTP diphosphokinase -
J6O05_RS05275 (1082809) 1082809..1083057 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
J6O05_RS05280 (1083057) 1083057..1083392 + 336 WP_000254739.1 endoribonuclease MazF Toxin
J6O05_RS05285 (1083463) 1083463..1084254 + 792 WP_057107940.1 nucleoside triphosphate pyrophosphohydrolase -
J6O05_RS05290 (1084482) 1084482..1086119 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
J6O05_RS05295 (1086207) 1086207..1087505 + 1299 WP_000036723.1 phosphopyruvate hydratase -
J6O05_RS05300 (1087649) 1087649..1087842 + 194 Protein_1039 transposase domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12126.09 Da        Isoelectric Point: 8.2618

>T200110 WP_000254739.1 NZ_CP073719:1083057-1083392 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVVPEELQLIKAKINVLIG

Download         Length: 336 bp

>T200110 NZ_CP097711:c1919777-1919403 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTTCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATCGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT200110 WP_000581937.1 NZ_CP073719:1082809-1083057 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT200110 NZ_CP097711:c1920234-1919866 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
ACAGCAATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References