Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 945886..946540 Replicon chromosome
Accession NZ_CP073719
Organism Escherichia coli strain CX12

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag J6O05_RS04700 Protein ID WP_000244777.1
Coordinates 946133..946540 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag J6O05_RS04695 Protein ID WP_000354046.1
Coordinates 945886..946152 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O05_RS04670 (941055) 941055..941798 + 744 WP_000951948.1 SDR family oxidoreductase -
J6O05_RS04675 (941855) 941855..943288 - 1434 WP_223566651.1 6-phospho-beta-glucosidase BglA -
J6O05_RS04680 (943333) 943333..943644 + 312 WP_001182947.1 N(4)-acetylcytidine aminohydrolase -
J6O05_RS04685 (943808) 943808..944467 + 660 WP_000250274.1 hemolysin III family protein -
J6O05_RS04690 (944663) 944663..945643 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
J6O05_RS04695 (945886) 945886..946152 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
J6O05_RS04700 (946133) 946133..946540 + 408 WP_000244777.1 protein YgfX Toxin
J6O05_RS04705 (946580) 946580..947101 - 522 WP_001055874.1 flavodoxin FldB -
J6O05_RS04710 (947213) 947213..948109 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
J6O05_RS04715 (948134) 948134..948844 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
J6O05_RS04720 (948850) 948850..950583 + 1734 WP_033816119.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T200109 WP_000244777.1 NZ_CP073719:946133-946540 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T200109 NZ_CP097711:1900650-1900904 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCAGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT200109 WP_000354046.1 NZ_CP073719:945886-946152 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT200109 NZ_CP097711:1900402-1900653 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCCCTGGAGGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCTGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References