200108

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type IV Classification (family/domain) yeeV-yafW/CbtA-CbeA
Location 828473..829266 Replicon chromosome
Accession NZ_CP073719
Organism Escherichia coli strain CX12

Toxin (Protein)


Gene name yeeV Uniprot ID S1EZ92
Locus tag J6O05_RS04095 Protein ID WP_000854726.1
Coordinates 828473..828850 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID S1EBQ7
Locus tag J6O05_RS04100 Protein ID WP_001548930.1
Coordinates 828940..829266 (-) Length 109 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O05_RS04075 (825213) 825213..826361 + 1149 WP_000287217.1 DUF3322 domain-containing protein -
J6O05_RS04080 (826852) 826852..827694 - 843 WP_001548928.1 DUF4942 domain-containing protein -
J6O05_RS04085 (827779) 827779..827976 - 198 WP_000839269.1 DUF957 domain-containing protein -
J6O05_RS04090 (827988) 827988..828476 - 489 WP_001547769.1 DUF5983 family protein -
J6O05_RS04095 (828473) 828473..828850 - 378 WP_000854726.1 TA system toxin CbtA family protein Toxin
J6O05_RS04100 (828940) 828940..829266 - 327 WP_001548930.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
J6O05_RS04105 (829285) 829285..829506 - 222 WP_000691994.1 DUF987 domain-containing protein -
J6O05_RS04110 (829515) 829515..829991 - 477 WP_000811693.1 RadC family protein -
J6O05_RS04115 (830007) 830007..830465 - 459 WP_000211838.1 antirestriction protein -
J6O05_RS04120 (830563) 830563..830802 - 240 WP_000194654.1 DUF905 family protein -
J6O05_RS04125 (830879) 830879..831346 - 468 WP_001548932.1 protein YkfB -
J6O05_RS04130 (831370) 831370..831813 - 444 WP_000824224.1 hypothetical protein -
J6O05_RS04135 (831813) 831813..832049 - 237 WP_001115854.1 protein YpjK -
J6O05_RS04140 (832086) 832086..832787 - 702 WP_001548933.1 WYL domain-containing protein -
J6O05_RS04145 (833005) 833005..833826 - 822 WP_001548934.1 DUF932 domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(1-112)

Antitoxin

(6-103)


Sequences


Toxin        


Download         Length: 126 a.a.        Molecular weight: 14164.10 Da        Isoelectric Point: 7.8045

>T200108 WP_000854726.1 NZ_CP073719:c828850-828473 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Download         Length: 378 bp

>T200108 NZ_CP097711:1900402-1900653 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCCCTGGAGGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCTGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 12301.97 Da        Isoelectric Point: 7.0273

>AT200108 WP_001548930.1 NZ_CP073719:c829266-828940 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTFGSCGYVYLAVYPTPETKK

Download         Length: 327 bp

>AT200108 NZ_CP097711:1900650-1900904 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCAGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB S1EZ92


Antitoxin

Source ID Structure
AlphaFold DB S1EBQ7

References