Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 263008..263639 Replicon chromosome
Accession NZ_CP073719
Organism Escherichia coli strain CX12

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag J6O05_RS01215 Protein ID WP_001259385.1
Coordinates 263008..263283 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag J6O05_RS01220 Protein ID WP_000593555.1
Coordinates 263280..263639 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O05_RS01200 (258012) 258012..259079 + 1068 WP_000361491.1 HlyD family efflux transporter periplasmic adaptor subunit -
J6O05_RS01205 (259076) 259076..261811 + 2736 WP_032294472.1 ribosome-associated ATPase/putative transporter RbbA -
J6O05_RS01210 (261811) 261811..262935 + 1125 Protein_239 ABC transporter permease -
J6O05_RS01215 (263008) 263008..263283 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
J6O05_RS01220 (263280) 263280..263639 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
J6O05_RS01225 (263759) 263759..264160 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
J6O05_RS01230 (264166) 264166..264972 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
J6O05_RS01235 (264969) 264969..265733 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
J6O05_RS01240 (265733) 265733..266566 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
J6O05_RS01245 (266563) 266563..267507 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 253785..263639 9854


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T200105 WP_001259385.1 NZ_CP073719:263008-263283 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T200105 NZ_CP097711:c833990-833613 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTTCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATCGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAATATTATCCTGGGTAAGCACCCGGAGGTGAAACAATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT200105 WP_000593555.1 NZ_CP073719:263280-263639 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT200105 NZ_CP097711:c834447-834079 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
ACAGCAATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References