200094

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 3801116..3801795 Replicon chromosome
Accession NZ_CP073718
Organism Escherichia coli strain CX08

Toxin (Protein)


Gene name ykfI Uniprot ID S1FHS4
Locus tag J6O57_RS18940 Protein ID WP_000854680.1
Coordinates 3801454..3801795 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID S1EWR7
Locus tag J6O57_RS18935 Protein ID WP_000070396.1
Coordinates 3801116..3801433 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O57_RS18890 (3796554) 3796554..3797375 + 822 WP_000197387.1 DUF932 domain-containing protein -
J6O57_RS18895 (3797592) 3797592..3798293 + 702 Protein_3699 WYL domain-containing protein -
J6O57_RS18900 (3798334) 3798334..3798570 + 237 WP_223566506.1 protein YpjK -
J6O57_RS18905 (3798570) 3798570..3799013 + 444 WP_000649865.1 hypothetical protein -
J6O57_RS18910 (3799036) 3799036..3799503 + 468 WP_001385283.1 protein YkfB -
J6O57_RS18915 (3799580) 3799580..3799819 + 240 WP_000194654.1 DUF905 family protein -
J6O57_RS18920 (3799917) 3799917..3800375 + 459 Protein_3704 antirestriction protein -
J6O57_RS18925 (3800391) 3800391..3800867 + 477 WP_000811693.1 RadC family protein -
J6O57_RS18930 (3800876) 3800876..3801097 + 222 WP_000691994.1 DUF987 domain-containing protein -
J6O57_RS18935 (3801116) 3801116..3801433 + 318 WP_000070396.1 type IV toxin-antitoxin system antitoxin YafW Antitoxin
J6O57_RS18940 (3801454) 3801454..3801795 + 342 WP_000854680.1 type IV toxin-antitoxin system toxin YkfI Toxin
J6O57_RS18945 (3802038) 3802038..3802735 + 698 WP_094096600.1 IS1-like element IS1A family transposase -
J6O57_RS18955 (3803144) 3803144..3804397 - 1254 WP_057108326.1 glutamate-5-semialdehyde dehydrogenase -
J6O57_RS18960 (3804409) 3804409..3805512 - 1104 WP_001285288.1 glutamate 5-kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA / rhs/PAAR / vgrG/tssI / hcp2/tssD2 / tssA / hcp1/tssD1 3778703..3844066 65363


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12892.95 Da        Isoelectric Point: 9.6543

>T200094 WP_000854680.1 NZ_CP073718:3801454-3801795 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T200094 NZ_CP097709:4075120-4075698 [Pseudomonas aeruginosa]
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAACGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAACATAGCTGGCGGCTGTGCCTGATGGCACTGGCCTTCGAGGACCAAC
TGGCCGGCCTGGACCTCGGCAAGGTCCTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCC
GCCGTCGAGCAGGCCGCGCACCCGGACAAAGGCGAACAGGAACGCGCCGACCTGTTGCAACTGACCCGTCACCTCGACGC
GCCGCTGCGCGACCGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGTCCGCCGAAGCCCTGGCAGTGAAGGCCC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGACAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGACCCTGCGCGAGCTACTCGACGCCAGGACCCGACAGCGCCTCGC
AGCTCGGCGCGACGCCTAG

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11982.62 Da        Isoelectric Point: 7.0270

>AT200094 WP_000070396.1 NZ_CP073718:3801116-3801433 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT200094 NZ_CP097709:4074477-4075031 [Pseudomonas aeruginosa]
ATGACCGAGCCTCTCCCTTCTTCGTCCCTGGGCCCCGCCCTGCGTCGCTGGCGCCTGCTGCACCGGGTCAAGCAAACGCA
TGCCGCCGAATTGCTCGGCGTGGCGCAATCGACCATCTCGCGCTGGGAGTCCGGTACCCAGGCGCTGCTGGTGGAGGAAC
GGGCACGCCTGGAGCGTCTGCTCGGCGCCCGCCTGGAAGCCGCCGCCGACCAGGCACTGGCCCGCCTGGTCGAGGCCAAC
CCGCAACCCGTGCACCTGATCTGCGACCTCACCCATCGCCTGTTGGCCTGTTCGCCGGCACGCGCCGCGCAATTCGGCGT
GCCTCTCGGCGAACTGCTCGACCGTTCGCTGTGGCCCTATTGCAGCGAGGAAATCCTCCGCCAGGAAGCCACCCTCGAGG
AACTCGGCTGGCGCGAACTGCTGGCACCGCCGGCGCTGGAGTTCGCCAGCGGAGCGAACGCCTCGGCCATCGTGCCGATC
CGGCGCAGCCGTTGCCGCTGGACCCGCATGACCCTCTCGGACGGACGCGCGGTGCGCCTGGTGGAAACCCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1FHS4

Antitoxin

Source ID Structure
AlphaFold DB S1EWR7

References