Detailed information of TA system TA200092

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2633481..2634119
• Replicon: chromosome
• Accession: NZ_CP073718
• Organism: Escherichia coli strain CX08

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: J6O57_RS13140
• Protein ID: WP_000813794.1
• Coordinates: 2633943..2634119 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: J6O57_RS13135
• Protein ID: WP_001270286.1
• Coordinates: 2633481..2633897 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O57_RS13115 (2628633)	2628633..2629574	-	942	WP_001251313.1	ABC transporter permease	-
J6O57_RS13120 (2629575)	2629575..2630588	-	1014	WP_057108537.1	ABC transporter ATP-binding protein	-
J6O57_RS13125 (2630606)	2630606..2631751	-	1146	Protein_2571	ABC transporter substrate-binding protein	-
J6O57_RS13130 (2631996)	2631996..2633402	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
J6O57_RS13135 (2633481)	2633481..2633897	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
J6O57_RS13140 (2633943)	2633943..2634119	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
J6O57_RS13145 (2634341)	2634341..2634571	+	231	WP_000494244.1	YncJ family protein	-
J6O57_RS13150 (2634663)	2634663..2636624	-	1962	WP_001488038.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
J6O57_RS13155 (2636697)	2636697..2637233	-	537	WP_000429169.1	DNA-binding transcriptional regulator SutR	-
J6O57_RS13160 (2637325)	2637325..2638500	+	1176	WP_263294461.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	sodB / mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA / espR1 / rhs/PAAR / vgrG/tssI	2243379..2679464	436085

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T200092 WP_000813794.1 NZ_CP073718:c2634119-2633943 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T200092 NZ_CP097709:2488983-2489441 [Pseudomonas aeruginosa]
ATGCAACGGCATGGCTGGACGCTGCTCTTCCACGACTGCGTGATCGAGCAGTTGCAGAAACTGCATGCGGCCGCGAGGCG
CGCGCAGGAGAATGACCCGGCGGGCTTCGAGTCCAATGCCAACGTCAAGCTCTTCCGGGCCCTGAGCCAGTTGATGTTGG
ATGTGGTGCCAGGCGATCCGGCACGCGACGAGTACCGCCAGGGCAACACCTTGGGACCTGCCCACCGCCACTGGCGAAGG
GCCAAGATCGGACGGCGGTTCCGGCTGTTCTTCCGGTACGACTCGAAGGCGAAGGTCATTGTGTACGCCTGGGTCAACGA
TGAACAGACCCTGCGGTCTTCGGGTAGCAAATCGGACCCGTATGTCGTGTTCGAGAAGATGCTCGGACGCGGGAACCCAC
CAGACGACTGGCACGCGCTGATACAGGCAAGCAAGCAGGATTGGAGCAAACTGGAATAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT200092 WP_001270286.1 NZ_CP073718:c2633897-2633481 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT200092 NZ_CP097709:2488666-2488983 [Pseudomonas aeruginosa]
ATGCCTGCCATCCACGAAGTCGCCACGCTGACCTCTAAAGGCCAGATCACGCTGCCCAAGCCCATCCGGCAGGCGCTCGG
CGTCGATGCCGGCGGCAAGCTCGCGTTCGATCTGCGGGGCAGCGAAGTCGTCGTCACCCGTGTTGATGCCGAGCACGAGG
ACCCCGCCATTGGCGCGTTTCTGAGCCTGCTGGCCCGCGACATCGAAGCTGGCCGGAACGTCCGGGGCCTGCCCGAGGAT
CTGGCGCGCGCCATGCTGGAGCATGCTGGCCACGGTGTGAACCTGGACGAGGAGATCGACGGGAAAGTGGAACTCTGA

Structures

Toxin

• PDB: 6HPB