Detailed information of TA system TA200078

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeV-yafW/CbtA-CbeA
• Location: 828473..829266
• Replicon: chromosome
• Accession: NZ_CP073718
• Organism: Escherichia coli strain CX08

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1EZ92
• Locus tag: J6O57_RS04105
• Protein ID: WP_000854726.1
• Coordinates: 828473..828850 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EBQ7
• Locus tag: J6O57_RS04110
• Protein ID: WP_001548930.1
• Coordinates: 828940..829266 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O57_RS04085 (825213)	825213..826361	+	1149	WP_000287217.1	DUF3322 domain-containing protein	-
J6O57_RS04090 (826852)	826852..827694	-	843	WP_001548928.1	DUF4942 domain-containing protein	-
J6O57_RS04095 (827779)	827779..827976	-	198	WP_000839269.1	DUF957 domain-containing protein	-
J6O57_RS04100 (827988)	827988..828476	-	489	WP_001547769.1	DUF5983 family protein	-
J6O57_RS04105 (828473)	828473..828850	-	378	WP_000854726.1	TA system toxin CbtA family protein	Toxin
J6O57_RS04110 (828940)	828940..829266	-	327	WP_001548930.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
J6O57_RS04115 (829285)	829285..829506	-	222	WP_000691994.1	DUF987 domain-containing protein	-
J6O57_RS04120 (829515)	829515..829991	-	477	WP_000811693.1	RadC family protein	-
J6O57_RS04125 (830007)	830007..830465	-	459	WP_000211838.1	antirestriction protein	-
J6O57_RS04130 (830563)	830563..830802	-	240	WP_000194654.1	DUF905 family protein	-
J6O57_RS04135 (830879)	830879..831346	-	468	WP_001548932.1	protein YkfB	-
J6O57_RS04140 (831370)	831370..831813	-	444	WP_000824224.1	hypothetical protein	-
J6O57_RS04145 (831813)	831813..832049	-	237	WP_001115854.1	protein YpjK	-
J6O57_RS04150 (832086)	832086..832787	-	702	WP_001548933.1	WYL domain-containing protein	-
J6O57_RS04155 (833005)	833005..833826	-	822	WP_001548934.1	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14164.10 Da
• Isoelectric Point: 7.8045

>T200078 WP_000854726.1 NZ_CP073718:c828850-828473 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T200078 NZ_CP097703:299205-299573 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12301.97 Da
• Isoelectric Point: 7.0273

>AT200078 WP_001548930.1 NZ_CP073718:c829266-828940 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTFGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 327 bp

>AT200078 NZ_CP097703:298987-299208 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: S1EZ92

Antitoxin

• AlphaFold DB: S1EBQ7