Detailed information of TA system TA200064

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3376571..3377191
• Replicon: chromosome
• Accession: NZ_CP073715
• Organism: Salmonella enterica subsp. enterica serovar Bovismorbificans strain 2020LSAL11867

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: V1H8E6
• Locus tag: KCG42_RS16480
• Protein ID: WP_001280991.1
• Coordinates: 3376973..3377191 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: V1H4V6
• Locus tag: KCG42_RS16475
• Protein ID: WP_000344807.1
• Coordinates: 3376571..3376945 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KCG42_RS16465 (3371710)	3371710..3372903	+	1194	WP_001039202.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
KCG42_RS16470 (3372926)	3372926..3376075	+	3150	WP_001132508.1	efflux RND transporter permease AcrB	-
KCG42_RS16475 (3376571)	3376571..3376945	+	375	WP_000344807.1	Hha toxicity modulator TomB	Antitoxin
KCG42_RS16480 (3376973)	3376973..3377191	+	219	WP_001280991.1	HHA domain-containing protein	Toxin
KCG42_RS16485 (3377370)	3377370..3377921	+	552	WP_001278792.1	maltose O-acetyltransferase	-
KCG42_RS16490 (3378039)	3378039..3378509	+	471	WP_000136183.1	YlaC family protein	-
KCG42_RS16495 (3378565)	3378565..3378705	-	141	WP_001197749.1	type B 50S ribosomal protein L36	-
KCG42_RS16500 (3378711)	3378711..3378971	-	261	WP_000801415.1	type B 50S ribosomal protein L31	-
KCG42_RS16505 (3379196)	3379196..3380746	+	1551	WP_024792601.1	EAL domain-containing protein	-
KCG42_RS16515 (3380977)	3380977..3381366	+	390	WP_000961285.1	MGMT family protein	-
KCG42_RS16520 (3381399)	3381399..3381968	-	570	WP_000779802.1	YbaY family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8613.97 Da
• Isoelectric Point: 8.9008

>T200064 WP_001280991.1 NZ_CP073715:3376973-3377191 [Salmonella enterica subsp. enterica serovar Bovismorbificans]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T200064 NZ_CP097697:329836-330204 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14453.27 Da
• Isoelectric Point: 5.1444

>AT200064 WP_000344807.1 NZ_CP073715:3376571-3376945 [Salmonella enterica subsp. enterica serovar Bovismorbificans]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT200064 NZ_CP097697:329618-329839 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• PDB: 4ICG

Antitoxin

• AlphaFold DB: A0A5I5FGI0