Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1676857..1677492 Replicon chromosome
Accession NZ_CP073714
Organism Paenibacillus pabuli strain E1

Toxin (Protein)


Gene name mazF Uniprot ID W4B4V0
Locus tag KET34_RS07385 Protein ID WP_024630714.1
Coordinates 1677142..1677492 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A2W6NI96
Locus tag KET34_RS07380 Protein ID WP_024630715.1
Coordinates 1676857..1677138 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KET34_RS07365 (KET34_07375) 1671955..1673448 - 1494 WP_247901304.1 serine hydrolase -
KET34_RS07370 (KET34_07380) 1673745..1674947 + 1203 WP_247901305.1 outer membrane lipoprotein-sorting protein -
KET34_RS07375 (KET34_07385) 1675408..1676595 + 1188 WP_247901306.1 alanine racemase -
KET34_RS07380 (KET34_07390) 1676857..1677138 + 282 WP_024630715.1 ribbon-helix-helix protein, CopG family Antitoxin
KET34_RS07385 (KET34_07395) 1677142..1677492 + 351 WP_024630714.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KET34_RS07390 (KET34_07400) 1677728..1679917 + 2190 WP_247901307.1 alpha-galactosidase -
KET34_RS07395 (KET34_07405) 1680192..1682414 + 2223 WP_282189451.1 Tex family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(9-46)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12778.77 Da        Isoelectric Point: 5.1184

>T200049 WP_024630714.1 NZ_CP073714:1677142-1677492 [Paenibacillus pabuli]
MIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTCIVAAITAQIQKAKLPTHVEIDAAAHGFDRDSVILLEQI
RTIDKQRLTDKITHLDEETMKLVNEALQISLGLIDF

Download         Length: 351 bp

>T200049 NZ_CP097694:328988-329356 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10723.33 Da        Isoelectric Point: 5.8649

>AT200049 WP_024630715.1 NZ_CP073714:1676857-1677138 [Paenibacillus pabuli]
VANLQNTKRIMISLPDYLLQEVDGIVALENSNRSELIRQAMKLYLTERKKRYIRESMQRGYMEMAKINLTMASEAFHAEE
DADSTLDRLVSGV

Download         Length: 282 bp

>AT200049 NZ_CP097694:328770-328991 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W4B4V0

Antitoxin

Source ID Structure
AlphaFold DB A0A2W6NI96

References