Detailed information of TA system TA200046

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2502818..2503383
• Replicon: chromosome
• Accession: NZ_CP073711
• Organism: Lacticaseibacillus rhamnosus strain X253

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: C2JYM4
• Locus tag: KEM50_RS11625
• Protein ID: WP_005692155.1
• Coordinates: 2502818..2503165 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: KEM50_RS11630
• Protein ID: WP_020752297.1
• Coordinates: 2503165..2503383 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEM50_RS11600 (KEM50_11600)	2498313..2499554	-	1242	WP_005692160.1	acyltransferase	-
KEM50_RS11605 (KEM50_11605)	2499551..2500252	-	702	WP_005692159.1	ATP-binding cassette domain-containing protein	-
KEM50_RS11610 (KEM50_11610)	2500245..2501063	-	819	WP_014571588.1	ABC transporter permease subunit	-
KEM50_RS11615 (KEM50_11615)	2501304..2501972	-	669	WP_005692157.1	phenylalanine--tRNA ligase beta subunit-related protein	-
KEM50_RS11625 (KEM50_11625)	2502818..2503165	-	348	WP_005692155.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KEM50_RS11630 (KEM50_11630)	2503165..2503383	-	219	WP_020752297.1	hypothetical protein	Antitoxin
KEM50_RS11635 (KEM50_11635)	2503477..2505312	-	1836	WP_015764721.1	ABC transporter ATP-binding protein	-
KEM50_RS11640 (KEM50_11640)	2505299..2507089	-	1791	WP_015764722.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-90)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13150.01 Da
• Isoelectric Point: 7.9817

>T200046 WP_005692155.1 NZ_CP073711:c2503165-2502818 [Lacticaseibacillus rhamnosus]
MTYKAKQGDVVWLDFDPSEGHEIRKRCPAVVLSSNAYNRATGFVIVAPITSTIRTLPGYVDIVSNKIRGQVVTSQVYSLD
VTEQGNRKIDFIERMNIEDFYTVAQFTKMNFDFKF

Nucleotide

• Length: 348 bp

>T200046 NZ_CP097692:c5417474-5417091 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8117.33 Da
• Isoelectric Point: 5.7995

>AT200046 WP_020752297.1 NZ_CP073711:c2503383-2503165 [Lacticaseibacillus rhamnosus]
MMDVKVVKRGNSLALSLPSSAGFKRGEAFLLISDEKGGYLLIPKVKNPYTKAKPLSFHQEEAWPDFDYEDIE

Nucleotide

• Length: 219 bp

>AT200046 NZ_CP097692:c5417716-5417474 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A7Z9DS75