Detailed information of TA system TA200044

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 471329..471982
• Replicon: chromosome
• Accession: NZ_CP073708
• Organism: Brevibacillus composti strain FJAT-54424

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KDJ56_RS02540
• Protein ID: WP_198828445.1
• Coordinates: 471632..471982 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: KDJ56_RS02535
• Protein ID: WP_198828444.1
• Coordinates: 471329..471628 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KDJ56_RS02500 (KDJ56_02500)	466369..466704	+	336	WP_198828437.1	metalloregulator ArsR/SmtB family transcription factor	-
KDJ56_RS02505 (KDJ56_02505)	466728..466979	-	252	WP_198828438.1	glutaredoxin family protein	-
KDJ56_RS02510 (KDJ56_02510)	467178..467348	+	171	WP_198828439.1	gamma-type small acid-soluble spore protein	-
KDJ56_RS02515 (KDJ56_02515)	467466..467864	+	399	WP_198828440.1	holo-ACP synthase	-
KDJ56_RS02520 (KDJ56_02520)	468123..469157	+	1035	WP_198828441.1	DUF4367 domain-containing protein	-
KDJ56_RS02525 (KDJ56_02525)	469521..469961	+	441	WP_198828442.1	hypothetical protein	-
KDJ56_RS02530 (KDJ56_02530)	469965..471167	+	1203	WP_198828443.1	alanine racemase	-
KDJ56_RS02535 (KDJ56_02535)	471329..471628	+	300	WP_198828444.1	CopG family ribbon-helix-helix protein	Antitoxin
KDJ56_RS02540 (KDJ56_02540)	471632..471982	+	351	WP_198828445.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KDJ56_RS02545 (KDJ56_02545)	472258..472602	+	345	WP_228728270.1	anti-sigma regulatory factor	-
KDJ56_RS02550 (KDJ56_02550)	472611..473630	+	1020	WP_198828447.1	PP2C family protein-serine/threonine phosphatase	-
KDJ56_RS02555 (KDJ56_02555)	473632..473958	+	327	WP_198828448.1	STAS domain-containing protein	-
KDJ56_RS02560 (KDJ56_02560)	473958..474446	+	489	WP_198828449.1	anti-sigma B factor RsbW	-
KDJ56_RS02565 (KDJ56_02565)	474415..475203	+	789	WP_198828450.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (15-52)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12961.98 Da
• Isoelectric Point: 4.8553

>T200044 WP_198828445.1 NZ_CP073708:471632-471982 [Brevibacillus composti]
VIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKMYGFDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMERVNESLQISLGIIDF

Nucleotide

• Length: 351 bp

>T200044 NZ_CP097692:c4937144-4936860 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11288.17 Da
• Isoelectric Point: 10.2102

>AT200044 WP_198828444.1 NZ_CP073708:471329-471628 [Brevibacillus composti]
MVGGGFVLSKSNTKRIMISLPHHLLQEVDGVIQKEKSNRSEFIRQAMHLYLKERKKRAIRETMQKGYMEMAKINLNMASE
AFGAEEEADHTLVRLVSGV

Nucleotide

• Length: 300 bp

>AT200044 NZ_CP097692:c4937376-4937134 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA