Detailed information of TA system TA200042

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 18488..19137
• Replicon: plasmid unnamed3
• Accession: NZ_CP073707
• Organism: Burkholderia cenocepacia strain toggle3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KEH57_RS36505
• Protein ID: WP_175914695.1
• Coordinates: 18488..18898 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A103EQ81
• Locus tag: KEH57_RS36510
• Protein ID: WP_059569609.1
• Coordinates: 18895..19137 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEH57_RS36465 (KEH57_36465)	14474..14815	-	342	WP_212267508.1	hypothetical protein	-
KEH57_RS36470 (KEH57_36470)	14862..15539	-	678	WP_212267506.1	hypothetical protein	-
KEH57_RS36475 (KEH57_36475)	15549..15803	-	255	WP_212267503.1	hypothetical protein	-
KEH57_RS36480 (KEH57_36480)	15839..16456	-	618	WP_212267499.1	hypothetical protein	-
KEH57_RS36485 (KEH57_36485)	16525..16779	-	255	WP_212267496.1	hypothetical protein	-
KEH57_RS36490 (KEH57_36490)	16916..17407	+	492	WP_212273250.1	hypothetical protein	-
KEH57_RS36495 (KEH57_36495)	17475..17864	-	390	WP_212273252.1	hypothetical protein	-
KEH57_RS36500 (KEH57_36500)	18214..18414	-	201	WP_212267609.1	hypothetical protein	-
KEH57_RS36505 (KEH57_36505)	18488..18898	-	411	WP_175914695.1	type II toxin-antitoxin system VapC family toxin	Toxin
KEH57_RS36510 (KEH57_36510)	18895..19137	-	243	WP_059569609.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
KEH57_RS37545	19624..19869	-	246	WP_249214461.1	hypothetical protein	-
KEH57_RS36520 (KEH57_36520)	20702..21379	+	678	WP_249216177.1	chromosome partitioning protein	-
KEH57_RS36525 (KEH57_36525)	21379..22344	+	966	WP_175914696.1	ParB/RepB/Spo0J family partition protein	-
KEH57_RS36530 (KEH57_36530)	23100..23957	+	858	WP_212267607.1	plasmid replication initiator TrfA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..134199	134199

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (12-50)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14828.14 Da
• Isoelectric Point: 5.8355

>T200042 WP_175914695.1 NZ_CP073707:c18898-18488 [Burkholderia cenocepacia]
MTLYLLDTNILSNVIRDPRGACATRIGQMPPEQVCTSIIVAAELRFGVWKRGSSTLAQRVEQLLASLTVLPLQPDADRCY
GRLRADLEKQGQLIGANDMLIAAHALAVDAVLVTDNTAEFTRVSGLPVENWLRPAT

Nucleotide

• Length: 411 bp

>T200042 NZ_CP097692:4220074-4220292 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9350.59 Da
• Isoelectric Point: 4.6856

>AT200042 WP_059569609.1 NZ_CP073707:c19137-18895 [Burkholderia cenocepacia]
MPTHDTRHARLFRNGRSQAVRIPREFELPVSEVTIHREGRRLIIEPVEAAPLRELFAQWTPLDETFPDIEDLPPEPVDIE

Nucleotide

• Length: 243 bp

>AT200042 NZ_CP097692:4219673-4220047 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A103EQ81