Detailed information of TA system TA200041

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 2311886..2313112
• Replicon: chromosome
• Accession: NZ_CP073704
• Organism: Burkholderia cenocepacia strain toggle3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A364GRG0
• Locus tag: KEH57_RS32390
• Protein ID: WP_006498847.1
• Coordinates: 2312531..2313112 (+)
• Length: 194 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KEH57_RS32385
• Protein ID: WP_058903402.1
• Coordinates: 2311886..2312452 (+)
• Length: 189 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEH57_RS32360 (KEH57_32360)	2307279..2307914	+	636	WP_059721935.1	isochorismatase family cysteine hydrolase	-
KEH57_RS32365 (KEH57_32365)	2307940..2308671	-	732	WP_027811744.1	ribonuclease T2	-
KEH57_RS32370 (KEH57_32370)	2308707..2309291	+	585	WP_227739690.1	OsmC family protein	-
KEH57_RS32375 (KEH57_32375)	2309370..2309942	-	573	WP_059721933.1	hypothetical protein	-
KEH57_RS32380 (KEH57_32380)	2310036..2311439	-	1404	WP_059721932.1	hypothetical protein	-
KEH57_RS32385 (KEH57_32385)	2311886..2312452	+	567	WP_058903402.1	helix-turn-helix transcriptional regulator	Antitoxin
KEH57_RS32390 (KEH57_32390)	2312531..2313112	+	582	WP_006498847.1	HD domain-containing protein	Toxin
KEH57_RS32395 (KEH57_32395)	2313168..2313896	+	729	WP_006483165.1	class I SAM-dependent methyltransferase	-
KEH57_RS32400 (KEH57_32400)	2314168..2315361	+	1194	WP_006483156.1	efflux RND transporter periplasmic adaptor subunit	-
KEH57_RS32405 (KEH57_32405)	2315364..2317397	+	2034	WP_048986896.1	MacB family efflux pump subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-173)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-64)

Sequences

Toxin

Protein

• Length: 194 a.a.
• Molecular weight: 21312.88 Da
• Isoelectric Point: 4.9572

>T200041 WP_006498847.1 NZ_CP073704:2312531-2313112 [Burkholderia cenocepacia]
MNPDTIRARLDFLRDAERLKDVLRSGHTSAGRAESTAEHSWRLCLMALVFADALPGVDTLKLLKLCVVHDLGEALHGDIP
AIEQAAHPDKSAHERDDLLTLTASLDRAARDEIVALWDEYEAATSPEARAAKALDKLETILQHNQGSNPPDFDYAFNLGY
GRRYTDAAPLFSAIREIVDVDTQRRIDAGGQRA

Nucleotide

• Length: 582 bp

>T200041 NZ_CP097692:c2799916-2798594 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCTGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 189 a.a.
• Molecular weight: 20617.58 Da
• Isoelectric Point: 10.7753

>AT200041 WP_058903402.1 NZ_CP073704:2311886-2312452 [Burkholderia cenocepacia]
MPHPPLHPSSLGPQLKRWRALHRVKQSHAAELFRVAQSTISRWEAGLQQMSPAERATAERLLAARLDSAGDHALARLIAG
STGRMHLVCDFTHRLLASSPARAAEFSRPLSTLLGASLWRYATPEIARMEAALDPLGWYDRAGPPSVEFDTGANASRVVP
IRGSLCRWTRMTLSDGSAVRLVETLDAH

Nucleotide

• Length: 567 bp

>AT200041 NZ_CP097692:c2800182-2799916 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Toxin

• AlphaFold DB: A0A364GRG0