Detailed information of TA system TA200040

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 2138496..2139241
• Replicon: chromosome
• Accession: NZ_CP073704
• Organism: Burkholderia cenocepacia strain toggle3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KEH57_RS31680
• Protein ID: WP_212203318.1
• Coordinates: 2138496..2138981 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KEH57_RS31685
• Protein ID: WP_059722025.1
• Coordinates: 2139002..2139241 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEH57_RS31655 (KEH57_31655)	2134040..2134837	+	798	WP_125331693.1	AraC family transcriptional regulator	-
KEH57_RS31660 (KEH57_31660)	2135016..2135948	+	933	WP_080322496.1	DMT family transporter	-
KEH57_RS31665 (KEH57_31665)	2136152..2136700	+	549	WP_006485834.1	hypothetical protein	-
KEH57_RS31670 (KEH57_31670)	2136794..2136928	-	135	WP_006485835.1	entericidin A/B family lipoprotein	-
KEH57_RS31675 (KEH57_31675)	2137163..2138458	+	1296	WP_006485851.1	aspartate carbamoyltransferase	-
KEH57_RS31680 (KEH57_31680)	2138496..2138981	-	486	WP_212203318.1	VapC toxin family PIN domain ribonuclease	Toxin
KEH57_RS31685 (KEH57_31685)	2139002..2139241	-	240	WP_059722025.1	Arc family DNA-binding protein	Antitoxin
KEH57_RS31690 (KEH57_31690)	2139538..2141781	+	2244	WP_058903292.1	TonB-dependent siderophore receptor	-
KEH57_RS31695 (KEH57_31695)	2141785..2142540	+	756	WP_006485843.1	tetratricopeptide repeat protein	-
KEH57_RS31700 (KEH57_31700)	2142537..2143220	+	684	WP_058903293.1	Fe2+-dependent dioxygenase	-
KEH57_RS31705 (KEH57_31705)	2143289..2143528	+	240	WP_058903294.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
KEH57_RS31710 (KEH57_31710)	2143708..2143995	+	288	WP_256858346.1	M15 family metallopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Arc-like DNA binding domain (7-46)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 16960.68 Da
• Isoelectric Point: 10.9341

>T200040 WP_212203318.1 NZ_CP073704:c2138981-2138496 [Burkholderia cenocepacia]
MSEPLRREPNAAVIEWLDAQKVETLYLAAIGLAELRSGVAALPEGAGGIGCSRVSGSVCCRCFAVGSCRSTRRRAGRASL
CARTRAAGNAIAVADGYIAATAEANGSIVATRDVAPFQAFGPRVIDPCAVRFAIAGSFGVPKCKTRRAWRRVLNRTIRLV
P

Nucleotide

• Length: 486 bp

>T200040 NZ_CP097692:2068432-2068534 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8555.74 Da
• Isoelectric Point: 4.8228

>AT200040 WP_059722025.1 NZ_CP073704:c2139241-2139002 [Burkholderia cenocepacia]
MPVITVRNLPDEARRALRIRAARHGRSTEAEVRDILEQAVLPGGRLELGTLLAEIGREAGDVDIGVPRDETPAGPMGFE

Nucleotide

• Length: 240 bp

>AT200040 NZ_CP097692:c2068541-2068396 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT