Detailed information of TA system TA200033

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 3000912..3001444
• Replicon: chromosome
• Accession: NZ_CP073702
• Organism: Burkholderia cenocepacia strain toggle3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: KEH57_RS13955
• Protein ID: WP_212268290.1
• Coordinates: 3001121..3001444 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: KEH57_RS13950
• Protein ID: WP_012327974.1
• Coordinates: 3000912..3001124 (+)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEH57_RS13915 (KEH57_13915)	2996374..2997669	+	1296	WP_012492927.1	Tol-Pal system beta propeller repeat protein TolB	-
KEH57_RS13920 (KEH57_13920)	2997687..2998199	+	513	WP_006484980.1	peptidoglycan-associated lipoprotein Pal	-
KEH57_RS13925 (KEH57_13925)	2998219..2998968	+	750	WP_006484965.1	tol-pal system protein YbgF	-
KEH57_RS13935 (KEH57_13935)	2999395..2999715	-	321	WP_212268292.1	helix-turn-helix transcriptional regulator	-
KEH57_RS13940 (KEH57_13940)	2999856..3000251	+	396	WP_012327976.1	hypothetical protein	-
KEH57_RS13945 (KEH57_13945)	3000319..3000732	+	414	WP_249216097.1	hypothetical protein	-
KEH57_RS13950 (KEH57_13950)	3000912..3001124	+	213	WP_012327974.1	antitoxin MazE family protein	Antitoxin
KEH57_RS13955 (KEH57_13955)	3001121..3001444	+	324	WP_212268290.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KEH57_RS37170	3001971..3002288	+	318	WP_226245292.1	hypothetical protein	-
KEH57_RS13965 (KEH57_13965)	3002290..3003324	+	1035	WP_212268286.1	relaxase/mobilization nuclease domain-containing protein	-
KEH57_RS13970 (KEH57_13970)	3003447..3003890	-	444	WP_212268284.1	inner membrane protein YiaA	-
KEH57_RS13975 (KEH57_13975)	3003994..3004941	-	948	WP_212268281.1	AraC family transcriptional regulator	-
KEH57_RS13980 (KEH57_13980)	3005071..3005400	+	330	WP_006484989.1	carboxymuconolactone decarboxylase family protein	-
KEH57_RS13985 (KEH57_13985)	3005604..3006438	+	835	Protein_2750	class II glutamine amidotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (7-67)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11666.79 Da
• Isoelectric Point: 8.9366

>T200033 WP_212268290.1 NZ_CP073702:3001121-3001444 [Burkholderia cenocepacia]
MNRGSIVTVALQGDFGKARPALVVQSDLFAEHPSVTLLLMSSAMVDAPLIRITVVPSEQNGLRMPSQIAVDKMFTVRREK
IGRPIGHLEDELMVAINRSLLVFLGLA

Nucleotide

• Length: 324 bp

>T200033 NZ_CP097690:c140325-139882 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 7997.94 Da
• Isoelectric Point: 5.5492

>AT200033 WP_012327974.1 NZ_CP073702:3000912-3001124 [Burkholderia cenocepacia]
MAATTSERVRIHRENLRAAGLRPIQIWVPDVRQPGFADECARQSRMVHQSIDENDLLDFIEQATDLGHSQ

Nucleotide

• Length: 213 bp

>AT200033 NZ_CP097690:c140552-140322 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA