Detailed information of TA system TA200032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 2227392..2227926
• Replicon: chromosome
• Accession: NZ_CP073702
• Organism: Burkholderia cenocepacia strain toggle3

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: KEH57_RS10240
• Protein ID: WP_021033200.1
• Coordinates: 2227627..2227926 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: KEH57_RS10235
• Protein ID: WP_021033199.1
• Coordinates: 2227392..2227637 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEH57_RS10220 (KEH57_10220)	2224101..2224940	+	840	WP_034201792.1	N(5)-hydroxyornithine transformylase PvdF	-
KEH57_RS10225 (KEH57_10225)	2224940..2225956	+	1017	WP_034201946.1	GNAT family N-acetyltransferase	-
KEH57_RS10230 (KEH57_10230)	2226114..2227277	+	1164	WP_059723573.1	M20 aminoacylase family protein	-
KEH57_RS10235 (KEH57_10235)	2227392..2227637	+	246	WP_021033199.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
KEH57_RS10240 (KEH57_10240)	2227627..2227926	+	300	WP_021033200.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KEH57_RS10245 (KEH57_10245)	2228017..2229321	-	1305	WP_125333102.1	cobyrinate a,c-diamide synthase	-
KEH57_RS10250 (KEH57_10250)	2229324..2229926	-	603	WP_006476035.1	cob(I)yrinic acid a,c-diamide adenosyltransferase	-
KEH57_RS10255 (KEH57_10255)	2229962..2230339	-	378	WP_006496449.1	cobalamin biosynthesis protein	-
KEH57_RS10260 (KEH57_10260)	2230336..2231066	-	731	Protein_2019	uroporphyrinogen-III C-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-94)

Antitoxin

• Antitoxin ParD (1-78)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11720.37 Da
• Isoelectric Point: 6.9677

>T200032 WP_021033200.1 NZ_CP073702:2227627-2227926 [Burkholderia cenocepacia]
MAVKGRQLRLTPLAECDLEDIWRYTAEHWSPEQAEHYYRDLIGTMEALARGKKAGRICVVRDGYFRYPAGSHVVFYRETD
ETIDVIRVLHQRMDIERHL

Nucleotide

• Length: 300 bp

>T200032 NZ_CP097690:59407-59877 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8708.68 Da
• Isoelectric Point: 7.3196

>AT200032 WP_021033199.1 NZ_CP073702:2227392-2227637 [Burkholderia cenocepacia]
MSRNTSVSLGDHFAEFVDAQVRSGRYGSASDVVRAGLRLLESHETQMRALQEALKAGEGSGTPKPFDSEAFLARMRATHG
G

Nucleotide

• Length: 246 bp

>AT200032 NZ_CP097690:58967-59410 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA