Detailed information of TA system TA200029

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 1451803..1452441
• Replicon: chromosome
• Accession: NZ_CP073683
• Organism: Paenibacillus sp. S25

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: H6CFY1
• Locus tag: KAI37_RS06250
• Protein ID: WP_007429335.1
• Coordinates: 1452091..1452441 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H6CFY0
• Locus tag: KAI37_RS06245
• Protein ID: WP_007429334.1
• Coordinates: 1451803..1452084 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KAI37_RS06225 (KAI37_01263)	1446807..1447799	-	993	WP_220030926.1	ABC transporter substrate-binding protein	-
KAI37_RS06230 (KAI37_01264)	1448034..1448666	+	633	WP_220030927.1	LysE family transporter	-
KAI37_RS06235 (KAI37_01265)	1448777..1450054	+	1278	WP_076292444.1	outer membrane lipoprotein-sorting protein	-
KAI37_RS06240 (KAI37_01266)	1450313..1451509	+	1197	WP_028543050.1	alanine racemase	-
KAI37_RS06245 (KAI37_01267)	1451803..1452084	+	282	WP_007429334.1	ribbon-helix-helix protein, CopG family	Antitoxin
KAI37_RS06250 (KAI37_01268)	1452091..1452441	+	351	WP_007429335.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KAI37_RS06255 (KAI37_01269)	1452621..1454843	+	2223	WP_179085886.1	Tex family protein	-
KAI37_RS06260	1454948..1455082	-	135	WP_007429337.1	cortex morphogenetic protein CmpA	-
KAI37_RS06265 (KAI37_01270)	1455227..1455739	+	513	WP_071639939.1	SprT family protein	-
KAI37_RS06280 (KAI37_01273)	1456129..1457004	-	876	WP_220030928.1	Cof-type HAD-IIB family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12822.77 Da
• Isoelectric Point: 5.6195

>T200029 WP_007429335.1 NZ_CP073683:1452091-1452441 [Paenibacillus sp. S25]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAATHGFDRDSVVLLEQI
RTIDKQRLTDKITHLDEETMRKVSDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T200029 NZ_CP097688:c5474714-5474331 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10636.25 Da
• Isoelectric Point: 8.5257

>AT200029 WP_007429334.1 NZ_CP073683:1451803-1452084 [Paenibacillus sp. S25]
MANLQNTKRIMISLPDHLLQEVDGIVAMENSNRSEFIRQAMKLYVGERKKRYIREAMQRGYMEMAKINLTMASEAFHAEE
DANSTLGRTVSGV

Nucleotide

• Length: 282 bp

>AT200029 NZ_CP097688:c5474956-5474714 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: H6CFY1

Antitoxin

• AlphaFold DB: H6CFY0