Detailed information of TA system TA200028

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 1496040..1496678
• Replicon: chromosome
• Accession: NZ_CP073682
• Organism: Paenibacillus sp. S02

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: H6CFY1
• Locus tag: KAI36_RS06510
• Protein ID: WP_007429335.1
• Coordinates: 1496328..1496678 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H6CFY0
• Locus tag: KAI36_RS06505
• Protein ID: WP_007429334.1
• Coordinates: 1496040..1496321 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KAI36_RS06485 (KAI36_01308)	1491044..1492036	-	993	WP_025722055.1	ABC transporter substrate-binding protein	-
KAI36_RS06490 (KAI36_01309)	1492271..1492903	+	633	WP_220027298.1	LysE family transporter	-
KAI36_RS06495 (KAI36_01310)	1493014..1494291	+	1278	WP_025722053.1	outer membrane lipoprotein-sorting protein	-
KAI36_RS06500 (KAI36_01311)	1494550..1495746	+	1197	WP_220027299.1	alanine racemase	-
KAI36_RS06505 (KAI36_01312)	1496040..1496321	+	282	WP_007429334.1	ribbon-helix-helix protein, CopG family	Antitoxin
KAI36_RS06510 (KAI36_01313)	1496328..1496678	+	351	WP_007429335.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KAI36_RS06515 (KAI36_01314)	1496857..1499079	+	2223	WP_220027300.1	Tex family protein	-
KAI36_RS06520	1499184..1499318	-	135	WP_007429337.1	cortex morphogenetic protein CmpA	-
KAI36_RS06525 (KAI36_01315)	1499463..1499975	+	513	WP_071639939.1	SprT family protein	-
KAI36_RS06540 (KAI36_01318)	1500365..1501240	-	876	WP_220027301.1	Cof-type HAD-IIB family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12822.77 Da
• Isoelectric Point: 5.6195

>T200028 WP_007429335.1 NZ_CP073682:1496328-1496678 [Paenibacillus sp. S02]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAATHGFDRDSVVLLEQI
RTIDKQRLTDKITHLDEETMRKVSDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T200028 NZ_CP097688:c5150141-5149224 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10636.25 Da
• Isoelectric Point: 8.5257

>AT200028 WP_007429334.1 NZ_CP073682:1496040-1496321 [Paenibacillus sp. S02]
MANLQNTKRIMISLPDHLLQEVDGIVAMENSNRSEFIRQAMKLYVGERKKRYIREAMQRGYMEMAKINLTMASEAFHAEE
DANSTLGRTVSGV

Nucleotide

• Length: 282 bp

>AT200028 NZ_CP097688:c5150905-5150147 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGTCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Toxin

• AlphaFold DB: H6CFY1

Antitoxin

• AlphaFold DB: H6CFY0