Detailed information of TA system TA200027

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 274516..275155
• Replicon: plasmid p1
• Accession: NZ_CP073678
• Organism: Cupriavidus sp. KK10

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KB879_RS32455
• Protein ID: WP_211983554.1
• Coordinates: 274739..275155 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KB879_RS32450
• Protein ID: WP_211983553.1
• Coordinates: 274516..274752 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KB879_RS32430 (KB879_32430)	271537..272772	+	1236	WP_211983551.1	tyrosine-type recombinase/integrase	-
KB879_RS32435 (KB879_32435)	272846..273315	+	470	Protein_266	ProQ/FinO family protein	-
KB879_RS32440 (KB879_32440)	273521..273733	-	213	WP_249221465.1	helix-turn-helix transcriptional regulator	-
KB879_RS32445 (KB879_32445)	274178..274396	+	219	WP_249221466.1	tyrosine-type recombinase/integrase	-
KB879_RS32450 (KB879_32450)	274516..274752	+	237	WP_211983553.1	DNA-binding protein	Antitoxin
KB879_RS32455 (KB879_32455)	274739..275155	+	417	WP_211983554.1	type II toxin-antitoxin system VapC family toxin	Toxin
KB879_RS32465 (KB879_32465)	277171..278454	-	1284	WP_249221529.1	IS4 family transposase	-
KB879_RS32470 (KB879_32470)	279224..279682	+	459	WP_249221568.1	reverse transcriptase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB / katA / adeG	1..1670509	1670509

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15398.67 Da
• Isoelectric Point: 6.4881

>T200027 WP_211983554.1 NZ_CP073678:274739-275155 [Cupriavidus sp. KK10]
VYLVDTNVISEVRKRLRANKGVLAFFRQAVKKDDGLYLSVVTVGELRRGVEVVRHRGDEQQAAMLDAWLETVLSDFAQNI
LSVDEAVGQVWGRLRVPHPEHALDKLIAATALIHDLTVVTRNVEDFAGLGVRVLNPFD

Nucleotide

• Length: 417 bp

>T200027 NZ_CP097688:c4992261-4991977 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8464.60 Da
• Isoelectric Point: 5.6864

>AT200027 WP_211983553.1 NZ_CP073678:274516-274752 [Cupriavidus sp. KK10]
MANLLVRNVDEALVQRLREAAAAHGRSAEAEHREILAKALSGTKRKSFAEVLMSMPNVGEDADFARIDDVGDVARVFG

Nucleotide

• Length: 237 bp

>AT200027 NZ_CP097688:c4992493-4992251 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA