Detailed information of TA system TA200026

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 123024..123589
• Replicon: plasmid p1
• Accession: NZ_CP073678
• Organism: Cupriavidus sp. KK10

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KB879_RS31760
• Protein ID: WP_211983406.1
• Coordinates: 123024..123395 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KB879_RS31765
• Protein ID: WP_211983407.1
• Coordinates: 123392..123589 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KB879_RS31745 (KB879_31745)	118187..119209	-	1023	WP_211983405.1	hypothetical protein	-
KB879_RS31750 (KB879_31750)	119227..121890	-	2664	WP_211984273.1	DEAD/DEAH box helicase	-
KB879_RS39620	122627..122674	+	48	Protein_128	hypothetical protein	-
KB879_RS40265	122739..122861	+	123	WP_256449358.1	hypothetical protein	-
KB879_RS31760 (KB879_31760)	123024..123395	-	372	WP_211983406.1	type II toxin-antitoxin system VapC family toxin	Toxin
KB879_RS31765 (KB879_31765)	123392..123589	-	198	WP_211983407.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KB879_RS31770 (KB879_31770)	123613..123954	-	342	WP_211983408.1	hypothetical protein	-
KB879_RS39625	124235..124363	+	129	Protein_133	transcriptional regulator	-
KB879_RS31775 (KB879_31775)	124953..125228	-	276	WP_211983409.1	XRE family transcriptional regulator	-
KB879_RS31780 (KB879_31780)	125641..126678	-	1038	WP_211983410.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
KB879_RS31785 (KB879_31785)	126671..127264	-	594	WP_211984277.1	DUF6088 family protein	-
KB879_RS31790 (KB879_31790)	127557..128261	+	705	WP_211983411.1	translesion DNA synthesis-associated protein ImuA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB / katA / adeG	1..1670509	1670509

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-44)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13626.79 Da
• Isoelectric Point: 4.8728

>T200026 WP_211983406.1 NZ_CP073678:c123395-123024 [Cupriavidus sp. KK10]
MILVDTSVWIDHINASDPVLISLLAEERVLAHPYVIGEISLSSLRDRDVVLGALLDLPRAPVVTPEETFYLIEREGLFNR
GIGYVDTSLLASARLQPGITIWTRDKRLKKVADELNLGAVLAH

Nucleotide

• Length: 372 bp

>T200026 NZ_CP097688:c4888154-4887621 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7116.22 Da
• Isoelectric Point: 10.6605

>AT200026 WP_211983407.1 NZ_CP073678:c123589-123392 [Cupriavidus sp. KK10]
MRTTIALDDDLIAKAQAYTGLEEKTALVREALKALIQREAAKRLANLGGSQPGIKGAPRRRRDVE

Nucleotide

• Length: 198 bp

>AT200026 NZ_CP097688:c4888431-4888165 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA