Detailed information of TA system TA200021

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ParST/RES-TIGR02293
• Location: 2298046..2299031
• Replicon: chromosome
• Accession: NZ_CP073677
• Organism: Cupriavidus sp. KK10

Toxin (Protein)

• Gene name: ParT
• Uniprot ID: -
• Locus tag: KB879_RS11075
• Protein ID: WP_211981292.1
• Coordinates: 2298558..2299031 (+)
• Length: 158 a.a.

Antitoxin (Protein)

• Gene name: ParS
• Uniprot ID: A0A022FWI5
• Locus tag: KB879_RS11070
• Protein ID: WP_035823930.1
• Coordinates: 2298046..2298561 (+)
• Length: 172 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KB879_RS11040 (KB879_11040)	2293479..2294123	+	645	WP_153955736.1	TetR/AcrR family transcriptional regulator	-
KB879_RS11045 (KB879_11045)	2294438..2294755	+	318	WP_153956217.1	hypothetical protein	-
KB879_RS11050 (KB879_11050)	2294833..2295015	+	183	WP_153956216.1	hypothetical protein	-
KB879_RS11055 (KB879_11055)	2295067..2295492	-	426	WP_035823937.1	hypothetical protein	-
KB879_RS11060 (KB879_11060)	2295742..2296869	-	1128	WP_153956215.1	site-specific integrase	-
KB879_RS11065 (KB879_11065)	2296983..2297948	+	966	WP_211981287.1	DNA-binding protein	-
KB879_RS11070 (KB879_11070)	2298046..2298561	+	516	WP_035823930.1	DUF2384 domain-containing protein	Antitoxin
KB879_RS11075 (KB879_11075)	2298558..2299031	+	474	WP_211981292.1	RES family NAD+ phosphorylase	Toxin
KB879_RS11080 (KB879_11080)	2299035..2300612	-	1578	WP_211981293.1	fatty acid--CoA ligase	-
KB879_RS11085 (KB879_11085)	2300808..2303117	+	2310	WP_211981296.1	ATP-dependent DNA helicase	-
KB879_RS11090 (KB879_11090)	2303244..2303567	+	324	WP_211981298.1	thioredoxin family protein	-
KB879_RS11095 (KB879_11095)	2303585..2304016	-	432	WP_010809388.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (4-152)

Antitoxin

• Antitoxin Xre (36-171)

Sequences

Toxin

Protein

• Length: 158 a.a.
• Molecular weight: 16699.92 Da
• Isoelectric Point: 6.0730

>T200021 WP_211981292.1 NZ_CP073677:2298558-2299031 [Cupriavidus sp. KK10]
VRPAWRIATDTPDYTADDASGAGAKATGGRWNRQGTPLIYAASSIALACLETLVHLGAGGLPLNRYLVRIDIPDELWAAA
QQLSADTAPVGWDAIPAGKTSLDTGEAWTAAGTSALLLVPSIVIPEEHNVLVNPAHPDAARLAYVKLRRWHYDARLV

Nucleotide

• Length: 474 bp

>T200021 NZ_CP097688:778073-778558 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 172 a.a.
• Molecular weight: 18492.03 Da
• Isoelectric Point: 7.2047

>AT200021 WP_035823930.1 NZ_CP073677:2298046-2298561 [Cupriavidus sp. KK10]
MPLTLTPEGRYPLAHERPAPPELSVNNYVAVYEASLSTQGAMSVIRAIREGVPASDLTRLAESMGTTKEQLIKTLDLPRA
TVDRKARAHQNLSSEQSERVLGMARLVGQVQALVESSGDPRGFSAAQWVAQWLEQPSPALGGRRPAELMDTLAGQRIVSD
LVARMQSGAYA

Nucleotide

• Length: 516 bp

>AT200021 NZ_CP097688:777783-778076 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A022FWI5