Detailed information of TA system TA200019

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 218618..219095
• Replicon: chromosome
• Accession: NZ_CP073677
• Organism: Cupriavidus sp. KK10

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: G0ETC7
• Locus tag: KB879_RS01080
• Protein ID: WP_013955321.1
• Coordinates: 218826..219095 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: G0ETC6
• Locus tag: KB879_RS01075
• Protein ID: WP_013955320.1
• Coordinates: 218618..218842 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KB879_RS01050 (KB879_01050)	214656..215849	+	1194	WP_211980560.1	FAD-dependent oxidoreductase	-
KB879_RS01055 (KB879_01055)	215862..216059	+	198	WP_211982083.1	sulfur carrier protein ThiS	-
KB879_RS01060 (KB879_01060)	216063..216899	+	837	WP_010813326.1	thiazole synthase	-
KB879_RS01065 (KB879_01065)	216896..218032	+	1137	WP_211980561.1	thiamine phosphate synthase	-
KB879_RS01070 (KB879_01070)	218047..218565	+	519	WP_211980562.1	GNAT family N-acetyltransferase	-
KB879_RS01075 (KB879_01075)	218618..218842	+	225	WP_013955320.1	DUF6290 family protein	Antitoxin
KB879_RS01080 (KB879_01080)	218826..219095	+	270	WP_013955321.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KB879_RS01085 (KB879_01085)	219202..219654	+	453	WP_013955322.1	PaaI family thioesterase	-
KB879_RS01090 (KB879_01090)	219676..220110	-	435	WP_013955323.1	PGDYG domain-containing protein	-
KB879_RS01095 (KB879_01095)	220116..220943	-	828	WP_049800538.1	bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase	-
KB879_RS01100 (KB879_01100)	221240..222658	+	1419	WP_010813332.1	adenosylhomocysteinase	-
KB879_RS01105 (KB879_01105)	222730..223080	+	351	WP_013955325.1	phage holin family protein	-
KB879_RS01110 (KB879_01110)	223142..223972	+	831	WP_114131115.1	methylenetetrahydrofolate reductase [NAD(P)H]	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-88)

Antitoxin

• Relaxosome protein TraY (4-48)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10401.99 Da
• Isoelectric Point: 10.0391

>T200019 WP_013955321.1 NZ_CP073677:218826-219095 [Cupriavidus sp. KK10]
MAWRIEVTQTAEKQLAKLDRPVARRIVTFLREQVAASDDPRSLGEALKGSELGEYWKYRVGDWRLICQIEDARITVVVLR
LGNRRDVYR

Nucleotide

• Length: 270 bp

>T200019 NZ_CP097688:362113-362481 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8387.53 Da
• Isoelectric Point: 4.4066

>AT200019 WP_013955320.1 NZ_CP073677:218618-218842 [Cupriavidus sp. KK10]
MLAIRLPEDIEDRLEALAKRTGRTKTFYAREAIMQHLDDLEDLYLADKIAARVRAGEESTVTLDELEARLGLAD

Nucleotide

• Length: 225 bp

>AT200019 NZ_CP097688:361895-362116 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: G0ETC7

Antitoxin

• AlphaFold DB: G0ETC6