Detailed information of TA system TA200018

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 1962704..1963930
• Replicon: chromosome
• Accession: NZ_CP073676
• Organism: Burkholderia cenocepacia strain toggle4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A364GRG0
• Locus tag: KEH58_RS30715
• Protein ID: WP_006498847.1
• Coordinates: 1963349..1963930 (+)
• Length: 194 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KEH58_RS30710
• Protein ID: WP_077021268.1
• Coordinates: 1962704..1963270 (+)
• Length: 189 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEH58_RS30685 (KEH58_30685)	1958179..1958910	-	732	WP_027811744.1	ribonuclease T2	-
KEH58_RS30690 (KEH58_30690)	1958946..1959530	+	585	WP_227739690.1	OsmC family protein	-
KEH58_RS30695 (KEH58_30695)	1959609..1960085	-	477	WP_249212230.1	hypothetical protein	-
KEH58_RS30700 (KEH58_30700)	1960279..1961682	-	1404	WP_077056620.1	hypothetical protein	-
KEH58_RS36710	1962399..1962656	+	258	WP_249212231.1	hypothetical protein	-
KEH58_RS30710 (KEH58_30710)	1962704..1963270	+	567	WP_077021268.1	helix-turn-helix transcriptional regulator	Antitoxin
KEH58_RS30715 (KEH58_30715)	1963349..1963930	+	582	WP_006498847.1	HD domain-containing protein	Toxin
KEH58_RS30720 (KEH58_30720)	1963986..1964714	+	729	WP_006483165.1	class I SAM-dependent methyltransferase	-
KEH58_RS30725 (KEH58_30725)	1964986..1966179	+	1194	WP_006483156.1	efflux RND transporter periplasmic adaptor subunit	-
KEH58_RS30730 (KEH58_30730)	1966182..1968215	+	2034	WP_212137502.1	MacB family efflux pump subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-173)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-64)

Sequences

Toxin

Protein

• Length: 194 a.a.
• Molecular weight: 21312.88 Da
• Isoelectric Point: 4.9572

>T200018 WP_006498847.1 NZ_CP073676:1963349-1963930 [Burkholderia cenocepacia]
MNPDTIRARLDFLRDAERLKDVLRSGHTSAGRAESTAEHSWRLCLMALVFADALPGVDTLKLLKLCVVHDLGEALHGDIP
AIEQAAHPDKSAHERDDLLTLTASLDRAARDEIVALWDEYEAATSPEARAAKALDKLETILQHNQGSNPPDFDYAFNLGY
GRRYTDAAPLFSAIREIVDVDTQRRIDAGGQRA

Nucleotide

• Length: 582 bp

>T200018 NZ_CP097687:134780-135091 [Klebsiella pneumoniae]
ATGCACATTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA

Antitoxin

Protein

• Length: 189 a.a.
• Molecular weight: 20511.45 Da
• Isoelectric Point: 10.7753

>AT200018 WP_077021268.1 NZ_CP073676:1962704-1963270 [Burkholderia cenocepacia]
MTASPLHPSSLGPQLKRWRALHRVKQSHAAELFRVAQSTISRWEAGLQQMSPAERATAERLLAARLDSAGDHALARLIAG
STGRMHLVCDLTHRLLASSPARAAEFSRPLSTLLGASLWRYATPEIARMEAALDPLGWYDRAGPPSVEFDTGANASRVVP
IRGSLCRWTRMTLSDGSAVRLVETLDAH

Nucleotide

• Length: 567 bp

>AT200018 NZ_CP097687:135088-135507 [Klebsiella pneumoniae]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTCCTGATGGACCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA

Structures

Toxin

• AlphaFold DB: A0A364GRG0