Detailed information of TA system TA200016

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 1316013..1316652
• Replicon: chromosome
• Accession: NZ_CP073676
• Organism: Burkholderia cenocepacia strain toggle4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KEH58_RS27740
• Protein ID: WP_006481544.1
• Coordinates: 1316013..1316429 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A364H554
• Locus tag: KEH58_RS27745
• Protein ID: WP_006481537.1
• Coordinates: 1316416..1316652 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEH58_RS27715 (KEH58_27715)	1311120..1312220	-	1101	WP_200064134.1	nitronate monooxygenase	-
KEH58_RS27720 (KEH58_27720)	1312217..1313137	-	921	WP_006481536.1	enoyl-CoA hydratase	-
KEH58_RS27725 (KEH58_27725)	1313146..1313943	-	798	WP_006481539.1	hypothetical protein	-
KEH58_RS27730 (KEH58_27730)	1313954..1314847	-	894	WP_048988182.1	CoA-transferase	-
KEH58_RS27735 (KEH58_27735)	1314857..1315753	-	897	WP_058903174.1	VOC family protein	-
KEH58_RS27740 (KEH58_27740)	1316013..1316429	-	417	WP_006481544.1	type II toxin-antitoxin system VapC family toxin	Toxin
KEH58_RS27745 (KEH58_27745)	1316416..1316652	-	237	WP_006481537.1	DNA-binding protein	Antitoxin
KEH58_RS27750 (KEH58_27750)	1317145..1318371	+	1227	WP_212137838.1	acyltransferase	-
KEH58_RS27755 (KEH58_27755)	1318382..1319059	-	678	WP_077176171.1	hypothetical protein	-
KEH58_RS27760 (KEH58_27760)	1319708..1320481	-	774	WP_048988174.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15402.64 Da
• Isoelectric Point: 6.8818

>T200016 WP_006481544.1 NZ_CP073676:c1316429-1316013 [Burkholderia cenocepacia]
VFLIDTNVISEIRKGKRTNRGVRAFFRQAEADASPLYLSVVTVAELRRGVDLIRHRGDHSQASALEAWLTMILSGYAPNI
LPVDVETGQMWGHLRVPDPTHELDKLIAATALINDLTVVTRNVDDFARTGVRLLNPFE

Nucleotide

• Length: 417 bp

>T200016 NZ_CP097686:c50512-50096 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8710.78 Da
• Isoelectric Point: 5.0916

>AT200016 WP_006481537.1 NZ_CP073676:c1316652-1316416 [Burkholderia cenocepacia]
MANLLVRNVDDTLVQSLREQAAANGRSAEAEHRAILAEALGRPKRRTFAQVLMSMPEVGEDADFQRVQDSGEVRRVFD

Nucleotide

• Length: 237 bp

>AT200016 NZ_CP097686:c50739-50509 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A364H554