Detailed information of TA system TA200015

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 793989..794635
• Replicon: chromosome
• Accession: NZ_CP073676
• Organism: Burkholderia cenocepacia strain toggle4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KEH58_RS25480
• Protein ID: WP_109342384.1
• Coordinates: 794228..794635 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B4EGU8
• Locus tag: KEH58_RS25475
• Protein ID: WP_006491146.1
• Coordinates: 793989..794231 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEH58_RS25460 (KEH58_25460)	789985..791391	+	1407	WP_006491136.1	aspartate ammonia-lyase	-
KEH58_RS25465 (KEH58_25465)	791470..792975	+	1506	WP_006491141.1	amino acid permease	-
KEH58_RS25470 (KEH58_25470)	793047..793730	-	684	WP_080331177.1	phospholipase	-
KEH58_RS25475 (KEH58_25475)	793989..794231	+	243	WP_006491146.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KEH58_RS25480 (KEH58_25480)	794228..794635	+	408	WP_109342384.1	type II toxin-antitoxin system VapC family toxin	Toxin
KEH58_RS25485 (KEH58_25485)	795036..797411	+	2376	WP_112750658.1	MCP four helix bundle domain-containing protein	-
KEH58_RS36840 (KEH58_25490)	797434..798162	+	729	WP_034178465.1	methyl-accepting chemotaxis protein	-
KEH58_RS25495 (KEH58_25495)	798159..798740	+	582	WP_006486232.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

• SpoVT-AbrB domain (7-49)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14945.06 Da
• Isoelectric Point: 6.3763

>T200015 WP_109342384.1 NZ_CP073676:794228-794635 [Burkholderia cenocepacia]
MKFLLDTNAVIAILKGEPAILARLHAWHPADFGIPAIVAHELYYGAYKSQRAAANVARVDALQFEVVSFDTEDAQHAGEI
RAHLIAAGTPIGPYDALIAGQARARHLVLVTHNVREFERVPRLQFEDWLAEPRAD

Nucleotide

• Length: 408 bp

>T200015 NZ_CP097684:1828-2151 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGCAGACAGCCTCTAATAACCG
ATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8849.02 Da
• Isoelectric Point: 5.0197

>AT200015 WP_006491146.1 NZ_CP073676:793989-794231 [Burkholderia cenocepacia]
MDTAKLFKHGGSQAVRLPKDFRFDTAEVRIRRHGAAVILEPMPQDWAWLAPLIGPVDADFEAAATTQPSAQERSGLDVFE

Nucleotide

• Length: 243 bp

>AT200015 NZ_CP097684:1562-1840 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Structures

Antitoxin

• AlphaFold DB: B4EGU8