Detailed information of TA system TA200010

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 144590..145227
• Replicon: chromosome
• Accession: NZ_CP073674
• Organism: Burkholderia cenocepacia strain toggle4

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KEH58_RS00675
• Protein ID: WP_058902008.1
• Coordinates: 144826..145227 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A364GPY9
• Locus tag: KEH58_RS00670
• Protein ID: WP_034201144.1
• Coordinates: 144590..144826 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEH58_RS00650 (KEH58_00650)	139966..140982	+	1017	WP_006493582.1	helix-turn-helix domain-containing protein	-
KEH58_RS00655 (KEH58_00655)	141077..142174	-	1098	WP_212136668.1	ADP-heptose--LPS heptosyltransferase	-
KEH58_RS00660 (KEH58_00660)	142171..143874	-	1704	WP_212136670.1	thiamine pyrophosphate-binding protein	-
KEH58_RS00665 (KEH58_00665)	144243..144472	+	230	Protein_128	type II toxin-antitoxin system RelE/ParE family toxin	-
KEH58_RS00670 (KEH58_00670)	144590..144826	+	237	WP_034201144.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KEH58_RS00675 (KEH58_00675)	144826..145227	+	402	WP_058902008.1	type II toxin-antitoxin system VapC family toxin	Toxin
KEH58_RS00680 (KEH58_00680)	145294..146682	-	1389	WP_006485681.1	L-serine ammonia-lyase	-
KEH58_RS00685 (KEH58_00685)	146713..147843	-	1131	WP_085964363.1	alginate lyase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-120)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 15043.22 Da
• Isoelectric Point: 5.6634

>T200010 WP_058902008.1 NZ_CP073674:144826-145227 [Burkholderia cenocepacia]
MPRFMLDTNMCIYLMKNQPEQVARRFAQCYTGDVVMSAITYAELEYGLTACANPARERRHLAALIEDIPVAPFDAAAAQA
YGPVREATRERKKDHLDKLIAAHAVSLDVVLVTNNERDFVSYPGLRLENWLND

Nucleotide

• Length: 402 bp

>T200010 NZ_CP097683:4206519-4206737 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8982.15 Da
• Isoelectric Point: 6.4701

>AT200010 WP_034201144.1 NZ_CP073674:144590-144826 [Burkholderia cenocepacia]
MHTTRVFRNGNSQAVRIPADLAYERSDIELEIERVGDEIRIRPMRRPLTQVLAKFAKFGPDFMADGRGDHEQAEREGL

Nucleotide

• Length: 237 bp

>AT200010 NZ_CP097683:4206118-4206492 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A364GPY9