Detailed information of TA system TA200004

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 677484..678148
• Replicon: chromosome
• Accession: NZ_CP073670
• Organism: Burkholderia cenocepacia strain toggle2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A1V2W836
• Locus tag: KEH56_RS17175
• Protein ID: WP_059722920.1
• Coordinates: 677484..677780 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: KEH56_RS17180
• Protein ID: WP_059722922.1
• Coordinates: 677783..678148 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEH56_RS17160 (KEH56_17160)	673968..675093	-	1126	Protein_606	efflux RND transporter periplasmic adaptor subunit	-
KEH56_RS17165 (KEH56_17165)	675176..675793	-	618	WP_059722917.1	TetR/AcrR family transcriptional regulator	-
KEH56_RS17170 (KEH56_17170)	675871..677331	+	1461	WP_059722918.1	TolC family protein	-
KEH56_RS17175 (KEH56_17175)	677484..677780	+	297	WP_059722920.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KEH56_RS17180 (KEH56_17180)	677783..678148	+	366	WP_059722922.1	putative addiction module antidote protein	Antitoxin
KEH56_RS17185 (KEH56_17185)	678239..679818	-	1580	Protein_611	cytochrome c	-
KEH56_RS17190 (KEH56_17190)	679809..680210	-	402	WP_048987230.1	hypothetical protein	-
KEH56_RS17195 (KEH56_17195)	680207..682845	-	2639	Protein_613	cytochrome c oxidase subunit I	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Uncharacterized protein HI1420 (5-92)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10775.58 Da
• Isoelectric Point: 10.3618

>T200004 WP_059722920.1 NZ_CP073670:677484-677780 [Burkholderia cenocepacia]
MFKVLTTPQFDKWLGGLRDPVGSAAISLRIERAKLGNLGQWRAIGDGVNEMKIDVGPGYRAYFVRRGKIIVVVLCGGDKS
TQKKDIKTAKQIAAELED

Nucleotide

• Length: 297 bp

>T200004 NZ_CP097683:354859-355206 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 12903.57 Da
• Isoelectric Point: 8.5514

>AT200004 WP_059722922.1 NZ_CP073670:677783-678148 [Burkholderia cenocepacia]
MKISELAEFDGSKYLKDEEAIRHYLAQAFEDGDPRLIQAALGNVAKARGMTALARESGVKREALYRALSEGGNAEFATIM
KVVAALGLHLTVAPTTQNAEAGRGPEPTSRTRSHARAAAHG

Nucleotide

• Length: 366 bp

>AT200004 NZ_CP097683:355206-355505 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A1V2W836