Detailed information of TA system TA199998

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 379901..381127
• Replicon: chromosome
• Accession: NZ_CP073669
• Organism: Burkholderia cenocepacia strain toggle2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A364GRG0
• Locus tag: KEH56_RS01600
• Protein ID: WP_006498847.1
• Coordinates: 379901..380482 (-)
• Length: 194 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KEH56_RS01605
• Protein ID: WP_058903402.1
• Coordinates: 380561..381127 (-)
• Length: 189 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEH56_RS01585 (KEH56_01585)	375616..377649	-	2034	WP_048986896.1	MacB family efflux pump subunit	-
KEH56_RS01590 (KEH56_01590)	377652..378845	-	1194	WP_006483156.1	efflux RND transporter periplasmic adaptor subunit	-
KEH56_RS01595 (KEH56_01595)	379117..379845	-	729	WP_006483165.1	class I SAM-dependent methyltransferase	-
KEH56_RS01600 (KEH56_01600)	379901..380482	-	582	WP_006498847.1	HD domain-containing protein	Toxin
KEH56_RS01605 (KEH56_01605)	380561..381127	-	567	WP_058903402.1	helix-turn-helix transcriptional regulator	Antitoxin
KEH56_RS01610 (KEH56_01610)	381574..382977	+	1404	WP_059721932.1	hypothetical protein	-
KEH56_RS01615 (KEH56_01615)	383071..383643	+	573	WP_059721933.1	hypothetical protein	-
KEH56_RS01620 (KEH56_01620)	383722..384306	-	585	WP_227739690.1	OsmC family protein	-
KEH56_RS01625 (KEH56_01625)	384342..385073	+	732	WP_027811744.1	ribonuclease T2	-
KEH56_RS01630 (KEH56_01630)	385099..385734	-	636	WP_059721935.1	isochorismatase family cysteine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-173)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-64)

Sequences

Toxin

Protein

• Length: 194 a.a.
• Molecular weight: 21312.88 Da
• Isoelectric Point: 4.9572

>T199998 WP_006498847.1 NZ_CP073669:c380482-379901 [Burkholderia cenocepacia]
MNPDTIRARLDFLRDAERLKDVLRSGHTSAGRAESTAEHSWRLCLMALVFADALPGVDTLKLLKLCVVHDLGEALHGDIP
AIEQAAHPDKSAHERDDLLTLTASLDRAARDEIVALWDEYEAATSPEARAAKALDKLETILQHNQGSNPPDFDYAFNLGY
GRRYTDAAPLFSAIREIVDVDTQRRIDAGGQRA

Nucleotide

• Length: 582 bp

>T199998 NZ_CP097680:33336-33647 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 189 a.a.
• Molecular weight: 20617.58 Da
• Isoelectric Point: 10.7753

>AT199998 WP_058903402.1 NZ_CP073669:c381127-380561 [Burkholderia cenocepacia]
MPHPPLHPSSLGPQLKRWRALHRVKQSHAAELFRVAQSTISRWEAGLQQMSPAERATAERLLAARLDSAGDHALARLIAG
STGRMHLVCDFTHRLLASSPARAAEFSRPLSTLLGASLWRYATPEIARMEAALDPLGWYDRAGPPSVEFDTGANASRVVP
IRGSLCRWTRMTLSDGSAVRLVETLDAH

Nucleotide

• Length: 567 bp

>AT199998 NZ_CP097680:33644-34063 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: A0A364GRG0