Detailed information of TA system TA199997

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 100643..101289
• Replicon: plasmid unnamed3
• Accession: NZ_CP073668
• Organism: Burkholderia contaminans strain toggle1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KEH59_RS42105
• Protein ID: WP_009692912.1
• Coordinates: 100643..101050 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KEH59_RS42110
• Protein ID: WP_009692911.1
• Coordinates: 101047..101289 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEH59_RS42070 (KEH59_42070)	96424..97077	-	654	WP_226292009.1	hypothetical protein	-
KEH59_RS42075 (KEH59_42075)	97140..97448	-	309	WP_069301923.1	hypothetical protein	-
KEH59_RS43100	97465..97755	-	291	WP_069301924.1	hypothetical protein	-
KEH59_RS42085 (KEH59_42085)	97776..98675	-	900	WP_069301925.1	hypothetical protein	-
KEH59_RS42090 (KEH59_42090)	98672..99280	-	609	WP_226292010.1	hypothetical protein	-
KEH59_RS42095 (KEH59_42095)	99315..99626	-	312	WP_069301926.1	hypothetical protein	-
KEH59_RS42100 (KEH59_42100)	99970..100500	-	531	WP_141716966.1	hypothetical protein	-
KEH59_RS42105 (KEH59_42105)	100643..101050	-	408	WP_009692912.1	type II toxin-antitoxin system VapC family toxin	Toxin
KEH59_RS42110 (KEH59_42110)	101047..101289	-	243	WP_009692911.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
KEH59_RS43380	101276..101524	-	249	WP_252447792.1	hypothetical protein	-
KEH59_RS43385	101545..101895	-	351	WP_283140468.1	hypothetical protein	-
KEH59_RS43110	101921..102163	-	243	WP_226292011.1	hypothetical protein	-
KEH59_RS42120 (KEH59_42120)	103076..103762	+	687	WP_009692909.1	ParA family partition ATPase	-
KEH59_RS42125 (KEH59_42125)	103759..104802	+	1044	WP_069301928.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..164302	164302

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• SpoVT-AbrB domain (12-49)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14506.64 Da
• Isoelectric Point: 4.8230

>T199997 WP_009692912.1 NZ_CP073668:c101050-100643 [Burkholderia contaminans]
MSLYLLDTNILSNVIRDPRGACASRIGETPPEQVCTSIVVAAELRYGVCKRGSSALAQRVEQLLASLTVLPLQSDADQCY
GRLRADLERQGQLIGANDMLIAAHALAVDAVLVTDNTAEFTRVAGLAVENWLRPA

Nucleotide

• Length: 408 bp

>T199997 NZ_CP097679:c5365420-5365037 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9309.54 Da
• Isoelectric Point: 4.8615

>AT199997 WP_009692911.1 NZ_CP073668:c101289-101047 [Burkholderia contaminans]
MPTHDTRHARLFRNGRSQAVRIPREFELPVSEVTIHREGRRLIIEPVEAPSLRELFAQWAPLDETFPDIENLPAEPVDIE

Nucleotide

• Length: 243 bp

>AT199997 NZ_CP097679:c5365662-5365420 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA