Detailed information of TA system TA199996

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 87611..88269
• Replicon: plasmid unnamed3
• Accession: NZ_CP073668
• Organism: Burkholderia contaminans strain toggle1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KEH59_RS42005
• Protein ID: WP_069301955.1
• Coordinates: 87611..88042 (-)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KEH59_RS42010
• Protein ID: WP_069301954.1
• Coordinates: 88042..88269 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEH59_RS41970 (KEH59_41970)	83692..83865	-	174	WP_174990676.1	hypothetical protein	-
KEH59_RS41975 (KEH59_41975)	84011..84385	-	375	WP_069301959.1	hypothetical protein	-
KEH59_RS41980 (KEH59_41980)	84382..85122	-	741	WP_069301958.1	hypothetical protein	-
KEH59_RS41985 (KEH59_41985)	85137..86222	-	1086	WP_069301957.1	hypothetical protein	-
KEH59_RS41990 (KEH59_41990)	86270..86443	-	174	WP_174990675.1	hypothetical protein	-
KEH59_RS41995 (KEH59_41995)	86457..87167	-	711	WP_083264182.1	helix-turn-helix domain-containing protein	-
KEH59_RS42000 (KEH59_42000)	87263..87589	-	327	WP_141716973.1	hypothetical protein	-
KEH59_RS42005 (KEH59_42005)	87611..88042	-	432	WP_069301955.1	PIN domain-containing protein	Toxin
KEH59_RS42010 (KEH59_42010)	88042..88269	-	228	WP_069301954.1	CopG family transcriptional regulator	Antitoxin
KEH59_RS42015 (KEH59_42015)	88628..89836	+	1209	WP_070162840.1	hypothetical protein	-
KEH59_RS42020 (KEH59_42020)	89926..90168	+	243	WP_069301913.1	hypothetical protein	-
KEH59_RS42025 (KEH59_42025)	90294..90698	+	405	WP_141716963.1	hypothetical protein	-
KEH59_RS42030 (KEH59_42030)	90739..91308	+	570	WP_141716964.1	hypothetical protein	-
KEH59_RS42035 (KEH59_42035)	91305..91742	+	438	WP_069301916.1	hypothetical protein	-
KEH59_RS42040 (KEH59_42040)	91789..91929	-	141	WP_174990674.1	hypothetical protein	-
KEH59_RS42045 (KEH59_42045)	92038..92958	-	921	WP_069301917.1	hypothetical protein	-
KEH59_RS42050 (KEH59_42050)	92955..93236	-	282	WP_069301918.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..164302	164302

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-127)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15621.98 Da
• Isoelectric Point: 7.1847

>T199996 WP_069301955.1 NZ_CP073668:c88042-87611 [Burkholderia contaminans]
MTYLLDVNVLIALLDPAHVQHEPAHAWFGRVGYAAWSTCPLTENGVLRIIGNPKYPNTMGSPASVAPLVAQLRAHAGHTF
WPDDVSLLDEKHVDATRLLSSAQLTDTYLLALARVHKGKLATFDRRLVVDAVPNGAKHLELIS

Nucleotide

• Length: 432 bp

>T199996 NZ_CP097679:c4885087-4884803 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8066.16 Da
• Isoelectric Point: 10.6932

>AT199996 WP_069301954.1 NZ_CP073668:c88269-88042 [Burkholderia contaminans]
MRTTIDIDDDILALARARAKPGTSIGKVISELARAALQRTTTNQTSRNGLPLMPTSATARSVTLDTVNQLRDDSL

Nucleotide

• Length: 228 bp

>AT199996 NZ_CP097679:c4885319-4885077 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA