Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 86983..87530 Replicon plasmid pPNP1
Accession NZ_CP073662
Organism Pseudomonas sp. JS425

Toxin (Protein)


Gene name relE Uniprot ID A0A2J7Y1W0
Locus tag KDB76_RS29400 Protein ID WP_062378649.1
Coordinates 86983..87258 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A2J7Y1T8
Locus tag KDB76_RS29405 Protein ID WP_062378647.1
Coordinates 87258..87530 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KDB76_RS29385 (KDB76_29385) 82819..83079 + 261 WP_062378968.1 phosphopantetheine-binding protein -
KDB76_RS29390 (KDB76_29390) 83066..84259 + 1194 WP_062378653.1 aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme -
KDB76_RS29395 (KDB76_29395) 84367..86097 + 1731 WP_062378651.1 ABC transporter ATP-binding protein -
KDB76_RS29400 (KDB76_29400) 86983..87258 - 276 WP_062378649.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KDB76_RS29405 (KDB76_29405) 87258..87530 - 273 WP_062378647.1 ribbon-helix-helix domain-containing protein Antitoxin
KDB76_RS29410 (KDB76_29410) 87709..88671 + 963 WP_102886376.1 tyrosine-type recombinase/integrase -
KDB76_RS29415 (KDB76_29415) 89130..89983 + 854 WP_086005488.1 IS5 family transposase -
KDB76_RS29420 (KDB76_29420) 89987..91042 - 1056 Protein_107 DUF4113 domain-containing protein -
KDB76_RS29425 (KDB76_29425) 91076..91338 - 263 Protein_108 Tn3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul1 - 1..125508 125508


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(10-47)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10690.32 Da        Isoelectric Point: 8.5757

>T199987 WP_062378649.1 NZ_CP073662:c87258-86983 [Pseudomonas sp. JS425]
MQLQWTSKAVSDLARLYDFLALVNRPVAARTVQQLTAAPLTLLTNPRIGERLDEFEPRDVRRLLVAHYEIRYEIAGDTIF
LLRLWHSREDR

Download         Length: 276 bp

>T199987 NZ_CP097678:c269186-268716 [Klebsiella pneumoniae]
GTGATCTTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10231.58 Da        Isoelectric Point: 4.8782

>AT199987 WP_062378647.1 NZ_CP073662:c87530-87258 [Pseudomonas sp. JS425]
MTATAKTRSVTAHVPIELAERIDEIAERLERSKNWIVKQALFAWLDQEEERSRLTREALLDVDAGRVIDHQAVQAWAASL
DTETPLPVPR

Download         Length: 273 bp

>AT199987 NZ_CP097678:c269626-269183 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J7Y1W0

Antitoxin

Source ID Structure
AlphaFold DB A0A2J7Y1T8

References