Detailed information of TA system TA199987

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 86983..87530
• Replicon: plasmid pPNP1
• Accession: NZ_CP073662
• Organism: Pseudomonas sp. JS425

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A2J7Y1W0
• Locus tag: KDB76_RS29400
• Protein ID: WP_062378649.1
• Coordinates: 86983..87258 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2J7Y1T8
• Locus tag: KDB76_RS29405
• Protein ID: WP_062378647.1
• Coordinates: 87258..87530 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KDB76_RS29385 (KDB76_29385)	82819..83079	+	261	WP_062378968.1	phosphopantetheine-binding protein	-
KDB76_RS29390 (KDB76_29390)	83066..84259	+	1194	WP_062378653.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
KDB76_RS29395 (KDB76_29395)	84367..86097	+	1731	WP_062378651.1	ABC transporter ATP-binding protein	-
KDB76_RS29400 (KDB76_29400)	86983..87258	-	276	WP_062378649.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KDB76_RS29405 (KDB76_29405)	87258..87530	-	273	WP_062378647.1	ribbon-helix-helix domain-containing protein	Antitoxin
KDB76_RS29410 (KDB76_29410)	87709..88671	+	963	WP_102886376.1	tyrosine-type recombinase/integrase	-
KDB76_RS29415 (KDB76_29415)	89130..89983	+	854	WP_086005488.1	IS5 family transposase	-
KDB76_RS29420 (KDB76_29420)	89987..91042	-	1056	Protein_107	DUF4113 domain-containing protein	-
KDB76_RS29425 (KDB76_29425)	91076..91338	-	263	Protein_108	Tn3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul1	-	1..125508	125508

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (10-47)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10690.32 Da
• Isoelectric Point: 8.5757

>T199987 WP_062378649.1 NZ_CP073662:c87258-86983 [Pseudomonas sp. JS425]
MQLQWTSKAVSDLARLYDFLALVNRPVAARTVQQLTAAPLTLLTNPRIGERLDEFEPRDVRRLLVAHYEIRYEIAGDTIF
LLRLWHSREDR

Nucleotide

• Length: 276 bp

>T199987 NZ_CP097678:c269186-268716 [Klebsiella pneumoniae]
GTGATCTTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10231.58 Da
• Isoelectric Point: 4.8782

>AT199987 WP_062378647.1 NZ_CP073662:c87530-87258 [Pseudomonas sp. JS425]
MTATAKTRSVTAHVPIELAERIDEIAERLERSKNWIVKQALFAWLDQEEERSRLTREALLDVDAGRVIDHQAVQAWAASL
DTETPLPVPR

Nucleotide

• Length: 273 bp

>AT199987 NZ_CP097678:c269626-269183 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A2J7Y1W0

Antitoxin

• AlphaFold DB: A0A2J7Y1T8