Detailed information of TA system TA199986

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-DUF971
• Location: 31525..32123
• Replicon: plasmid pPNP1
• Accession: NZ_CP073662
• Organism: Pseudomonas sp. JS425

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: KDB76_RS29095
• Protein ID: WP_048388330.1
• Coordinates: 31821..32123 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: KDB76_RS29090
• Protein ID: WP_042857721.1
• Coordinates: 31525..31824 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KDB76_RS29060 (KDB76_29060)	27690..28121	-	432	WP_047306860.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
KDB76_RS29065 (KDB76_29065)	28260..29213	+	954	WP_212632300.1	non-homologous end-joining DNA ligase	-
KDB76_RS29070 (KDB76_29070)	29242..29517	+	276	WP_134286862.1	hypothetical protein	-
KDB76_RS29075 (KDB76_29075)	30626..30943	-	318	WP_152690486.1	hypothetical protein	-
KDB76_RS29080 (KDB76_29080)	30945..31166	-	222	WP_048388335.1	hypothetical protein	-
KDB76_RS29085 (KDB76_29085)	31179..31502	-	324	WP_048388333.1	hypothetical protein	-
KDB76_RS29090 (KDB76_29090)	31525..31824	-	300	WP_042857721.1	putative addiction module antidote protein	Antitoxin
KDB76_RS29095 (KDB76_29095)	31821..32123	-	303	WP_048388330.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KDB76_RS29100 (KDB76_29100)	32327..33106	+	780	WP_182330310.1	DUF4942 domain-containing protein	-
KDB76_RS29105 (KDB76_29105)	33260..33628	+	369	WP_047303037.1	hypothetical protein	-
KDB76_RS29110 (KDB76_29110)	33661..33978	+	318	WP_047303035.1	hypothetical protein	-
KDB76_RS29115 (KDB76_29115)	34035..34253	+	219	WP_047303033.1	hypothetical protein	-
KDB76_RS29120 (KDB76_29120)	34319..35410	+	1092	WP_200902862.1	hypothetical protein	-
KDB76_RS29125 (KDB76_29125)	35557..35799	+	243	WP_047303031.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul1	-	1..125508	125508

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-95)

Antitoxin

• Uncharacterized protein HI1420 (6-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11261.85 Da
• Isoelectric Point: 10.3903

>T199986 WP_048388330.1 NZ_CP073662:c32123-31821 [Pseudomonas sp. JS425]
MKTIKQTATYRNWERKLRDKQAKAIIAARVFRVAHGLLGDVQPVGQGISELRIHHGPGYRVYFQQRGNQLVLLLCGGDKS
SQARDIETAKALASQWSDDE

Nucleotide

• Length: 303 bp

>T199986 NZ_CP097678:c153150-152668 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10853.33 Da
• Isoelectric Point: 5.1084

>AT199986 WP_042857721.1 NZ_CP073662:c31824-31525 [Pseudomonas sp. JS425]
MKEQFYDYDPADALTDAESIAVFMEDAFETGDAAYIAKCLGVVARAKGMTALANESGLSREHLYRSFSDKGNPTLKTLLA
VMRAVGLDLTTRKHVEPVH

Nucleotide

• Length: 300 bp

>AT199986 NZ_CP097678:c153404-153138 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATTCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA