Detailed information of TA system TA199985

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 17572..18265
• Replicon: plasmid pPNP1
• Accession: NZ_CP073662
• Organism: Pseudomonas sp. JS425

Toxin (Protein)

• Gene name: tad
• Uniprot ID: N2IHR9
• Locus tag: KDB76_RS28975
• Protein ID: WP_003151133.1
• Coordinates: 17572..17949 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: N2IIN5
• Locus tag: KDB76_RS28980
• Protein ID: WP_001172026.1
• Coordinates: 17930..18265 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KDB76_RS28960 (KDB76_28960)	13451..13834	+	384	Protein_13	hypothetical protein	-
KDB76_RS28965 (KDB76_28965)	13765..16794	-	3030	WP_010799689.1	Tn3 family transposase	-
KDB76_RS28970 (KDB76_28970)	16778..17380	-	603	WP_010465829.1	recombinase family protein	-
KDB76_RS28975 (KDB76_28975)	17572..17949	+	378	WP_003151133.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KDB76_RS28980 (KDB76_28980)	17930..18265	+	336	WP_001172026.1	helix-turn-helix transcriptional regulator	Antitoxin
KDB76_RS28985 (KDB76_28985)	18280..18615	+	336	WP_000741275.1	hypothetical protein	-
KDB76_RS28990 (KDB76_28990)	18639..18965	+	327	WP_000091614.1	hypothetical protein	-
KDB76_RS28995 (KDB76_28995)	18962..19333	+	372	WP_023294894.1	hypothetical protein	-
KDB76_RS29000 (KDB76_29000)	19472..19807	-	336	WP_192576888.1	hypothetical protein	-
KDB76_RS29005 (KDB76_29005)	19808..20077	+	270	WP_192576887.1	hypothetical protein	-
KDB76_RS29010 (KDB76_29010)	20074..20424	+	351	WP_212632297.1	TrfB-related DNA-binding protein	-
KDB76_RS29015 (KDB76_29015)	20421..20828	+	408	WP_104833234.1	hypothetical protein	-
KDB76_RS29020 (KDB76_29020)	21061..21696	+	636	WP_047306864.1	recombinase family protein	-
KDB76_RS29025 (KDB76_29025)	22153..22707	+	555	WP_047306863.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul1	-	1..125508	125508

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-93)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13705.78 Da
• Isoelectric Point: 9.4693

>T199985 WP_003151133.1 NZ_CP073662:17572-17949 [Pseudomonas sp. JS425]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQELRNAKTNH

Nucleotide

• Length: 378 bp

>T199985 NZ_CP097677:c153306-152890 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12201.39 Da
• Isoelectric Point: 10.6058

>AT199985 WP_001172026.1 NZ_CP073662:17930-18265 [Pseudomonas sp. JS425]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Nucleotide

• Length: 336 bp

>AT199985 NZ_CP097677:c153533-153303 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A024ELN5

Antitoxin

• AlphaFold DB: A0A024EKI7