Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 3973419..3974302 Replicon chromosome
Accession NZ_CP073661
Organism Pseudomonas sp. JS425

Toxin (Protein)


Gene name - Uniprot ID A0A0C1HTG7
Locus tag KDB76_RS18440 Protein ID WP_015270851.1
Coordinates 3973419..3973856 (-) Length 146 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID F8G488
Locus tag KDB76_RS18445 Protein ID WP_013973163.1
Coordinates 3973853..3974302 (-) Length 150 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KDB76_RS18420 (KDB76_18420) 3968658..3969488 + 831 WP_230491506.1 IS3 family transposase -
KDB76_RS18425 (KDB76_18425) 3969789..3971018 - 1230 WP_013973159.1 acyl-CoA dehydrogenase -
KDB76_RS18430 (KDB76_18430) 3971159..3972085 + 927 WP_013973160.1 LysR family transcriptional regulator -
KDB76_RS18435 (KDB76_18435) 3972257..3973411 + 1155 WP_013973161.1 aminotransferase class V-fold PLP-dependent enzyme -
KDB76_RS18440 (KDB76_18440) 3973419..3973856 - 438 WP_015270851.1 RES family NAD+ phosphorylase Toxin
KDB76_RS18445 (KDB76_18445) 3973853..3974302 - 450 WP_013973163.1 DUF2384 domain-containing protein Antitoxin
KDB76_RS18450 (KDB76_18450) 3974401..3975054 - 654 WP_013973164.1 oxygen-insensitive NAD(P)H-dependent nitroreductase NfsB -
KDB76_RS18455 (KDB76_18455) 3975159..3976082 + 924 WP_038409932.1 LysR family transcriptional regulator -
KDB76_RS18460 (KDB76_18460) 3976130..3976960 + 831 WP_013973166.1 AraC family transcriptional regulator -
KDB76_RS18465 (KDB76_18465) 3977011..3977595 + 585 WP_013973167.1 LysE family translocator -
KDB76_RS18470 (KDB76_18470) 3977720..3978934 - 1215 WP_024087364.1 sugar transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-137)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(33-91)


Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15969.19 Da        Isoelectric Point: 5.1199

>T199984 WP_015270851.1 NZ_CP073661:c3973856-3973419 [Pseudomonas sp. JS425]
VILWRISAYADLSGTGGLRVSGRWHQAGRPVVYAATSPAGAMLEVLVHLEIDPEDFPTTMRLLRIELPDTVSQAQLPALQ
PGWSSQTELTRGLGNRFLDECSALLLPVPSAIMPSTTNYLFNPRHPQANSARLEVEDFTPDSRLF

Download         Length: 438 bp

>T199984 NZ_CP097677:49729-49878 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 150 a.a.        Molecular weight: 17004.59 Da        Isoelectric Point: 6.3405

>AT199984 WP_013973163.1 NZ_CP073661:c3974302-3973853 [Pseudomonas sp. JS425]
MLAEVLRDNGYHEYRARLHALLEIPELASDFEIHTRITHGFAATWLVKLTELGVFTPVERDQIIPLRTLKTRIERDQPLT
VEESDRLFRSAHITAMAEAVFGEAGKAKRWLSKPKERFSGLTPMQMLTTQQGTTQVEEMLLQIAEGFGL

Download         Length: 450 bp

>AT199984 NZ_CP097677:c49684-49625 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C1HTG7

Antitoxin

Source ID Structure
AlphaFold DB F8G488

References