Detailed information of TA system TA199982

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1246939..1247856
• Replicon: chromosome
• Accession: NZ_CP073656
• Organism: Bacillus velezensis strain Y816

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: KEC47_RS06320
• Protein ID: WP_057079950.1
• Coordinates: 1247110..1247856 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: KEC47_RS06315
• Protein ID: WP_003154807.1
• Coordinates: 1246939..1247109 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEC47_RS06280 (KEC47_06280)	1242151..1243740	+	1590	WP_077722100.1	hypothetical protein	-
KEC47_RS06285 (KEC47_06285)	1243753..1244178	+	426	WP_176283280.1	hypothetical protein	-
KEC47_RS06290 (KEC47_06290)	1244183..1244380	+	198	WP_071391956.1	XkdX family protein	-
KEC47_RS06295 (KEC47_06295)	1244437..1245198	+	762	WP_094247020.1	phage portal protein	-
KEC47_RS06300 (KEC47_06300)	1245250..1245513	+	264	WP_003154815.1	hemolysin XhlA family protein	-
KEC47_RS06305 (KEC47_06305)	1245527..1245790	+	264	WP_003154813.1	phage holin	-
KEC47_RS06310 (KEC47_06310)	1245804..1246682	+	879	WP_024085195.1	N-acetylmuramoyl-L-alanine amidase	-
KEC47_RS19160	1246717..1246842	-	126	WP_003154809.1	hypothetical protein	-
KEC47_RS06315 (KEC47_06315)	1246939..1247109	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
KEC47_RS06320 (KEC47_06320)	1247110..1247856	-	747	WP_057079950.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
KEC47_RS06325 (KEC47_06325)	1247961..1248959	-	999	WP_003154805.1	inorganic phosphate transporter	-
KEC47_RS06330 (KEC47_06330)	1248972..1249589	-	618	WP_003154804.1	DUF47 domain-containing protein	-
KEC47_RS06335 (KEC47_06335)	1249875..1251191	-	1317	WP_094247018.1	amino acid permease	-
KEC47_RS06340 (KEC47_06340)	1251515..1252465	+	951	WP_176283281.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1213266..1252465	39199

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29033.52 Da
• Isoelectric Point: 4.7755

>T199982 WP_057079950.1 NZ_CP073656:c1247856-1247110 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T199982 NZ_CP097675:c5035053-5034136 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT199982 WP_003154807.1 NZ_CP073656:c1247109-1246939 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT199982 NZ_CP097675:c5035817-5035059 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGTCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: I2HQ14