Detailed information of TA system TA199981

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 511165..511802
• Replicon: chromosome
• Accession: NZ_CP073656
• Organism: Bacillus velezensis strain Y816

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: KEC47_RS02445
• Protein ID: WP_003156187.1
• Coordinates: 511452..511802 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: KEC47_RS02440
• Protein ID: WP_003156188.1
• Coordinates: 511165..511446 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEC47_RS02420 (KEC47_02420)	507530..508129	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
KEC47_RS02425 (KEC47_02425)	508222..508587	+	366	WP_071392104.1	holo-ACP synthase	-
KEC47_RS02430 (KEC47_02430)	508752..509759	+	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
KEC47_RS02435 (KEC47_02435)	509876..511045	+	1170	WP_077721837.1	alanine racemase	-
KEC47_RS02440 (KEC47_02440)	511165..511446	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
KEC47_RS02445 (KEC47_02445)	511452..511802	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
KEC47_RS02450 (KEC47_02450)	511920..512741	+	822	WP_003156182.1	STAS domain-containing protein	-
KEC47_RS02455 (KEC47_02455)	512746..513111	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
KEC47_RS02460 (KEC47_02460)	513114..513515	+	402	WP_003156178.1	anti-sigma regulatory factor	-
KEC47_RS02465 (KEC47_02465)	513527..514534	+	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
KEC47_RS02470 (KEC47_02470)	514598..514927	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
KEC47_RS02475 (KEC47_02475)	514924..515406	+	483	WP_212102597.1	anti-sigma B factor RsbW	-
KEC47_RS02480 (KEC47_02480)	515372..516160	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
KEC47_RS02485 (KEC47_02485)	516160..516762	+	603	WP_003156170.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T199981 WP_003156187.1 NZ_CP073656:511452-511802 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T199981 NZ_CP097675:c4878236-4877952 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT199981 WP_003156188.1 NZ_CP073656:511165-511446 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT199981 NZ_CP097675:c4878468-4878226 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5