Detailed information of TA system TA199976

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbE-relB/ParE-YafN
• Location: 2518560..2519079
• Replicon: chromosome
• Accession: NZ_CP073638
• Organism: Burkholderia cepacia strain ZJ1

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: -
• Locus tag: KEC55_RS27770
• Protein ID: WP_282508304.1
• Coordinates: 2518798..2519079 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A108LKY6
• Locus tag: KEC55_RS27765
• Protein ID: WP_048023838.1
• Coordinates: 2518560..2518808 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEC55_RS27745 (KEC55_27690)	2514293..2515513	-	1221	WP_282508299.1	CoA transferase	-
KEC55_RS27750 (KEC55_27695)	2515594..2516376	-	783	WP_282508300.1	crotonase/enoyl-CoA hydratase family protein	-
KEC55_RS27755 (KEC55_27700)	2516511..2517278	+	768	WP_282508301.1	helix-turn-helix domain-containing protein	-
KEC55_RS27760 (KEC55_27705)	2517365..2518423	+	1059	WP_282508302.1	CaiB/BaiF CoA-transferase family protein	-
KEC55_RS27765 (KEC55_27710)	2518560..2518808	+	249	WP_048023838.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KEC55_RS27770 (KEC55_27715)	2518798..2519079	+	282	WP_282508304.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KEC55_RS27775 (KEC55_27720)	2519173..2519616	-	444	WP_011353771.1	PaaI family thioesterase	-
KEC55_RS27780 (KEC55_27725)	2519888..2521507	+	1620	WP_282508305.1	methyl-accepting chemotaxis protein	-
KEC55_RS27785 (KEC55_27730)	2521480..2522589	-	1110	WP_282508306.1	AGE family epimerase/isomerase	-
KEC55_RS27790 (KEC55_27735)	2522704..2523402	+	699	WP_282508307.1	MgtC/SapB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-81)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10979.90 Da
• Isoelectric Point: 10.7091

>T199976 WP_282508304.1 NZ_CP073638:2518798-2519079 [Burkholderia cepacia]
MTFELAFLEPALREWKKLDRTVRDQFKTKLAERLENPRIPSAKLHGHPDRYKIKLRSVGYRLVYEVRDAEVVVLVVAVGR
RERDAVYLAAMKR

Nucleotide

• Length: 282 bp

>T199976 NZ_CP097675:878958-879365 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8963.31 Da
• Isoelectric Point: 5.0636

>AT199976 WP_048023838.1 NZ_CP073638:2518560-2518808 [Burkholderia cepacia]
MPHTILASVTASVSELKRNPMGTVAAGEGFPVAILNHNEPAFYCIPAKTWEAMVERLEDIEDNALADSRAKEKVVKVKLN
DL

Nucleotide

• Length: 249 bp

>AT199976 NZ_CP097675:878711-878977 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A108LKY6