Detailed information of TA system TA199973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 772977..774739
• Replicon: chromosome
• Accession: NZ_CP073638
• Organism: Burkholderia cepacia strain ZJ1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: KEC55_RS19915
• Protein ID: WP_282509944.1
• Coordinates: 773408..774739 (+)
• Length: 444 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: KEC55_RS19910
• Protein ID: WP_282509942.1
• Coordinates: 772977..773405 (+)
• Length: 143 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEC55_RS19890 (KEC55_19840)	768445..769110	+	666	WP_282509934.1	cation diffusion facilitator family transporter	-
KEC55_RS19895 (KEC55_19845)	769293..770483	-	1191	WP_282509936.1	LuxR C-terminal-related transcriptional regulator	-
KEC55_RS19900 (KEC55_19850)	770765..771703	+	939	WP_282509938.1	alpha/beta hydrolase fold domain-containing protein	-
KEC55_RS19905 (KEC55_19855)	771700..772824	+	1125	WP_282509940.1	bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II	-
KEC55_RS19910 (KEC55_19860)	772977..773405	+	429	WP_282509942.1	helix-turn-helix transcriptional regulator	Antitoxin
KEC55_RS19915 (KEC55_19865)	773408..774739	+	1332	WP_282509944.1	type II toxin-antitoxin system HipA family toxin	Toxin
KEC55_RS19920 (KEC55_19870)	775091..776326	+	1236	WP_282509946.1	NADH:flavin oxidoreductase	-
KEC55_RS19925 (KEC55_19875)	776359..777474	-	1116	WP_282509948.1	VOC family protein	-
KEC55_RS19930 (KEC55_19880)	777832..779139	+	1308	WP_282509950.1	shikimate transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (157-407)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-64)

Sequences

Toxin

Protein

• Length: 444 a.a.
• Molecular weight: 48706.51 Da
• Isoelectric Point: 7.4840

>T199973 WP_282509944.1 NZ_CP073638:773408-774739 [Burkholderia cepacia]
MAARARHDRLDLWMNGIPVGYWEVRRGVERLVYLPGWLDDPQGRPLSLSLPFTPGNQPHQGAIVADYFDNLLPDSEPIRR
RIAQRYRLGSTAPFELLASIGRDCVGAIQMLPPDETPVDLEAIEGVTLDDAAVADVLRHATAAPLPGRAEPDVDLRLSIA
GAQEKTALLRQRNRWLRPSGSTPTTHIFKLPLGRVGNMQADMRTSVENEWLCSKLVAAYGLPVAPCEIGRFDDQKALIVE
RFDRRPSRDGSWILRLPQEDLCQATGTPSGAKYESDGGPGIDTIMGILANSADAAQDRRNFFVAQLVFWVLAAIDGHAKN
FSIAHLPGNTYRSTPLYDVLSAHPIIGTRRNQLPPRRARLAMAVCGKNRHYVIGEIQPRHWIAQGRRVGLTEDDVHAAMA
AVAARTEPAIAEVTAQIPADFPADVADTIFDGMRRQSRKLAGG

Nucleotide

• Length: 1332 bp

>T199973 NZ_CP097675:330342-330710 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 143 a.a.
• Molecular weight: 15102.11 Da
• Isoelectric Point: 11.6566

>AT199973 WP_282509942.1 NZ_CP073638:772977-773405 [Burkholderia cepacia]
MAFPVQTLTQLRPILVGFRKSAGLTQAQLAARLGVTQQSYAQLEANPSAVSIERLFKVLNTLGVRMTLDLGAPDESAEPA
DTDTGADARPAPRKPAANTRTPDATRGATTAKSRTGTTTGAARKRTATAAPRKTRVSKREDW

Nucleotide

• Length: 429 bp

>AT199973 NZ_CP097675:330124-330345 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA