Detailed information of TA system TA199967

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 157714..158351
• Replicon: chromosome
• Accession: NZ_CP073637
• Organism: Burkholderia cepacia strain ZJ1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KEC55_RS00670
• Protein ID: WP_282506331.1
• Coordinates: 157950..158351 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: KEC55_RS00665
• Protein ID: WP_176049249.1
• Coordinates: 157714..157950 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEC55_RS00650 (KEC55_00650)	153416..154450	+	1035	WP_282506328.1	helix-turn-helix domain-containing protein	-
KEC55_RS00655 (KEC55_00655)	154500..155597	-	1098	WP_282506329.1	ADP-heptose--LPS heptosyltransferase	-
KEC55_RS00660 (KEC55_00660)	155594..157297	-	1704	WP_282506330.1	thiamine pyrophosphate-binding protein	-
KEC55_RS00665 (KEC55_00665)	157714..157950	+	237	WP_176049249.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KEC55_RS00670 (KEC55_00670)	157950..158351	+	402	WP_282506331.1	type II toxin-antitoxin system VapC family toxin	Toxin
KEC55_RS00675 (KEC55_00675)	158439..159827	-	1389	WP_282506332.1	L-serine ammonia-lyase	-
KEC55_RS00680 (KEC55_00680)	159858..160994	-	1137	WP_282506333.1	alginate lyase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-119)

Antitoxin

• SpoVT-AbrB domain (7-46)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14941.96 Da
• Isoelectric Point: 4.7132

>T199967 WP_282506331.1 NZ_CP073637:157950-158351 [Burkholderia cepacia]
MPRYMLDTNMCIYLLKNQPEQVARRFAQCYTGDVVMSAITYAELEYGVTACTSPAQERSNLAALIEDIPVAPFDAVAAQA
YGSVREATRERKRDHLDKLIAAHAVSLDVVLVTNNERDFVSYPGVRLENWLND

Nucleotide

• Length: 402 bp

>T199967 NZ_CP097670:c5372962-5372579 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8974.12 Da
• Isoelectric Point: 5.1651

>AT199967 WP_176049249.1 NZ_CP073637:157714-157950 [Burkholderia cepacia]
MHTTRVFRNGNSQAVRIPADLAYERSDIELEIERIGDEIRIRPMRRPLTGVLEKFAKFGPDFMAEGRGDQEQADREGL

Nucleotide

• Length: 237 bp

>AT199967 NZ_CP097670:c5373204-5372962 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA