Detailed information of TA system TA199966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1224217..1225133
• Replicon: chromosome
• Accession: NZ_CP073635
• Organism: Bacillus amyloliquefaciens strain Ba13

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: KEF49_RS06165
• Protein ID: WP_065981257.1
• Coordinates: 1224387..1225133 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: KEF49_RS06160
• Protein ID: WP_003154807.1
• Coordinates: 1224217..1224387 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEF49_RS06130 (KEF49_06130)	1221083..1221463	+	381	WP_065981254.1	XkdW family protein	-
KEF49_RS06135 (KEF49_06135)	1221468..1221665	+	198	WP_013351962.1	XkdX family protein	-
KEF49_RS06140 (KEF49_06140)	1221713..1222474	+	762	WP_212101067.1	phage portal protein	-
KEF49_RS06145 (KEF49_06145)	1222528..1222791	+	264	WP_013351964.1	hemolysin XhlA family protein	-
KEF49_RS06150 (KEF49_06150)	1222805..1223068	+	264	WP_013351965.1	phage holin	-
KEF49_RS06155 (KEF49_06155)	1223082..1223960	+	879	WP_212101071.1	N-acetylmuramoyl-L-alanine amidase	-
KEF49_RS19635	1223995..1224120	-	126	WP_003154809.1	hypothetical protein	-
KEF49_RS06160 (KEF49_06160)	1224217..1224387	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
KEF49_RS06165 (KEF49_06165)	1224387..1225133	-	747	WP_065981257.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
KEF49_RS06170 (KEF49_06170)	1225241..1226239	-	999	WP_065981258.1	inorganic phosphate transporter	-
KEF49_RS06175 (KEF49_06175)	1226252..1226869	-	618	WP_003154804.1	DUF47 domain-containing protein	-
KEF49_RS06180 (KEF49_06180)	1227154..1228470	-	1317	WP_065981259.1	amino acid permease	-
KEF49_RS06185 (KEF49_06185)	1228794..1229744	+	951	WP_115997110.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1182758..1285683	102925

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29041.53 Da
• Isoelectric Point: 4.6839

>T199966 WP_065981257.1 NZ_CP073635:c1225133-1224387 [Bacillus amyloliquefaciens]
MLLFYQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQTRQYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T199966 NZ_CP097670:c5049445-5048528 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT199966 WP_003154807.1 NZ_CP073635:c1224387-1224217 [Bacillus amyloliquefaciens]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT199966 NZ_CP097670:c5050209-5049451 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGTCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: I2HQ14