Detailed information of TA system TA199965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 508575..509212
• Replicon: chromosome
• Accession: NZ_CP073635
• Organism: Bacillus amyloliquefaciens strain Ba13

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: KEF49_RS02410
• Protein ID: WP_003156187.1
• Coordinates: 508862..509212 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: KEF49_RS02405
• Protein ID: WP_003156188.1
• Coordinates: 508575..508856 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KEF49_RS02385 (KEF49_02385)	504936..505535	-	600	WP_013351092.1	rhomboid family intramembrane serine protease	-
KEF49_RS02390 (KEF49_02390)	505628..505993	+	366	WP_115998347.1	holo-ACP synthase	-
KEF49_RS19590	506158..507165	+	1008	WP_065980767.1	outer membrane lipoprotein carrier protein LolA	-
KEF49_RS02400 (KEF49_02400)	507287..508456	+	1170	WP_115998346.1	alanine racemase	-
KEF49_RS02405 (KEF49_02405)	508575..508856	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
KEF49_RS02410 (KEF49_02410)	508862..509212	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
KEF49_RS02415 (KEF49_02415)	509333..510154	+	822	WP_065980769.1	STAS domain-containing protein	-
KEF49_RS02420 (KEF49_02420)	510159..510524	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
KEF49_RS02425 (KEF49_02425)	510527..510928	+	402	WP_065980770.1	anti-sigma regulatory factor	-
KEF49_RS02430 (KEF49_02430)	510940..511947	+	1008	WP_013351098.1	PP2C family protein-serine/threonine phosphatase	-
KEF49_RS02435 (KEF49_02435)	512011..512340	+	330	WP_014469786.1	anti-sigma factor antagonist	-
KEF49_RS02440 (KEF49_02440)	512337..512819	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
KEF49_RS02445 (KEF49_02445)	512785..513573	+	789	WP_013351101.1	RNA polymerase sigma factor SigB	-
KEF49_RS02450 (KEF49_02450)	513573..514175	+	603	WP_065980772.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T199965 WP_003156187.1 NZ_CP073635:508862-509212 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T199965 NZ_CP097670:c4892628-4892344 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT199965 WP_003156188.1 NZ_CP073635:508575-508856 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT199965 NZ_CP097670:c4892860-4892618 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5