Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 53673..54409 Replicon plasmid unnamed1
Accession NZ_CP073628
Organism Klebsiella pneumoniae isolate KP_NORM_BLD_2012_85054

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag KD921_RS25780 Protein ID WP_003026803.1
Coordinates 53927..54409 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag KD921_RS25775 Protein ID WP_003026799.1
Coordinates 53673..53939 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KD921_RS25730 (KD921_25720) 49735..50097 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
KD921_RS25735 (KD921_25725) 50147..50497 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
KD921_RS25740 (KD921_25730) 50855..51124 + 270 WP_004152102.1 hypothetical protein -
KD921_RS25745 (KD921_25735) 51112..51687 + 576 WP_004152103.1 hypothetical protein -
KD921_RS25750 (KD921_25740) 51718..52212 + 495 WP_004152104.1 DNA-binding protein -
KD921_RS25755 (KD921_25745) 52256..52624 + 369 WP_004152105.1 hypothetical protein -
KD921_RS25760 (KD921_25750) 52658..52861 + 204 WP_004152106.1 HHA domain-containing protein -
KD921_RS25765 (KD921_25755) 52910..53167 + 258 WP_004152107.1 hypothetical protein -
KD921_RS25770 (KD921_25760) 53243..53497 + 255 WP_004152108.1 hypothetical protein -
KD921_RS25775 (KD921_25765) 53673..53939 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
KD921_RS25780 (KD921_25770) 53927..54409 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
KD921_RS25785 (KD921_25775) 54617..55963 + 1347 WP_077253535.1 ISNCY family transposase -
KD921_RS25790 (KD921_25780) 56012..56407 + 396 WP_004143398.1 helix-turn-helix domain-containing protein -
KD921_RS25795 (KD921_25785) 56555..57720 - 1166 Protein_59 IS3 family transposase -
KD921_RS25800 (KD921_25790) 57897..58859 - 963 WP_004152113.1 zinc metalloprotease HtpX -
KD921_RS25805 (KD921_25795) 58846..59334 - 489 WP_004152114.1 phosphate-starvation-inducible PsiE family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-206 / blaCTX-M-15 / dfrA14 / qnrB1 / tet(A) / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa - 1..247177 247177


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T199953 WP_003026803.1 NZ_CP073628:53927-54409 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T199953 NZ_CP097667:122457-122807 [Klebsiella pneumoniae]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT199953 WP_003026799.1 NZ_CP073628:53673-53939 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT199953 NZ_CP097667:122812-123114 [Klebsiella pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References