Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5202596..5203221 Replicon chromosome
Accession NZ_CP073627
Organism Klebsiella pneumoniae isolate KP_NORM_BLD_2012_85054

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KD921_RS25140 Protein ID WP_002882817.1
Coordinates 5202596..5202979 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KD921_RS25145 Protein ID WP_004150355.1
Coordinates 5202979..5203221 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KD921_RS25125 (5199962) 5199962..5200864 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KD921_RS25130 (5200861) 5200861..5201496 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KD921_RS25135 (5201493) 5201493..5202422 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KD921_RS25140 (5202596) 5202596..5202979 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KD921_RS25145 (5202979) 5202979..5203221 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KD921_RS25150 (5203426) 5203426..5204343 + 918 WP_009484979.1 alpha/beta hydrolase -
KD921_RS25155 (5204357) 5204357..5205298 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KD921_RS25160 (5205343) 5205343..5205780 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KD921_RS25165 (5205777) 5205777..5206637 - 861 WP_004146232.1 virulence factor BrkB family protein -
KD921_RS25170 (5206631) 5206631..5207230 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T199952 WP_002882817.1 NZ_CP073627:c5202979-5202596 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T199952 NZ_CP097666:c109212-109063 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT199952 WP_004150355.1 NZ_CP073627:c5203221-5202979 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT199952 NZ_CP097666:109257-109316 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References